Literature summary for 1.1.99.40 extracted from

Becker-Kettern, J.; Paczia, N.; Conrotte, J.F.; Kay, D.P.; Guignard, C.; Jung, P.P.; Linster, C.L.
Saccharomyces cerevisiae forms D-2-hydroxyglutarate and couples its degradation to D-lactate formation via a cytosolic transhydrogenase (2016), J. Biol. Chem., 291, 6036-6058.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.111	-	D-2-hydroxyglutarate	pH 9.5, 30°C	Saccharomyces cerevisiae
0.45	-	pyruvate	pH 9.5, 30°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Saccharomyces cerevisiae	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	0.005 mM, about 3fold stimulation	Saccharomyces cerevisiae
Zn2+	0.005 mM, about 3fold stimulation	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P39976	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein. Purified fractions show a yellow color	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + D-lactate	-	Saccharomyces cerevisiae	D-2-hydroxyglutarate + pyruvate	-	r
D-2-hydroxyglutarate + oxaloacetate	-	Saccharomyces cerevisiae	2-oxoglutarate + malate	-	r
D-2-hydroxyglutarate + pyruvate	-	Saccharomyces cerevisiae	2-oxoglutarate + D-lactate	-	r
additional information	no substrate: L-lactate. The enzyme displays dehydrogenase activity with artificial acceptor dichlorophenolindophenol	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
D-2-hydroxyglutarate-pyruvate transhydrogenase	-	Saccharomyces cerevisiae
DLD3	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4	-	D-2-hydroxyglutarate	pH 9.5, 30°C	Saccharomyces cerevisiae
4.9	-	pyruvate	pH 9.5, 30°C	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
FAD	enzyme is a FAD-dependent transhydrogenase using pyruvate as a hydrogen acceptor	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
physiological function	a mutant strain lacking cytosolic transhydrogenase Dld3 activity accumulates millimolar levels of D-2-hydroxyglutarate	Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
12	-	pyruvate	pH 9.5, 30°C	Saccharomyces cerevisiae
36	-	D-2-hydroxyglutarate	pH 9.5, 30°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)