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Literature summary for 1.1.99.6 extracted from
- Dehydrogenation of D-lactate by a soluble enzyme from kidney mitochondria (1959), Biochim. Biophys. Acta, 34, 290-291.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ferricyanide | - |
Oryctolagus cuniculus |  |
| methylene blue | - |
Oryctolagus cuniculus | |
| additional information | ageing of extracts increases dehydrogenase activity | Oryctolagus cuniculus | |
| O2 | no appreciable O2 uptake in absence of electron carrier | Oryctolagus cuniculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inhibition potentiated by KCN | Oryctolagus cuniculus | |
| Cu2+ | strong | Oryctolagus cuniculus | |
| EDTA | - |
Oryctolagus cuniculus | |
| Hg2+ | strong | Oryctolagus cuniculus | |
| KCN | potentiates inhibition of 1,10-phenanthroline | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | strong | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
D-lactate | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCN | raises dehydrogenase activity of freshly prepared kidney extracts | Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + acceptor | Oryctolagus cuniculus | enzyme acts on a variety of (R)-2-hydroxy acids | pyruvate + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Oryctolagus cuniculus | - |
| liver | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + acceptor | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | enzyme acts on a variety of (R)-2-hydroxy acids | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Oryctolagus cuniculus |
| 30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | - |
Oryctolagus cuniculus | |
| cytochrome c | possible physiological acceptor | Oryctolagus cuniculus | |
| phenazine methosulfate | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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