Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | 100 mM, 2.2fold increase in enzyme activity | Oryctolagus cuniculus | |
methylene blue | - |
Oryctolagus cuniculus | |
additional information | NAD+ or NADP+ do not act as acceptors | Oryctolagus cuniculus | |
O2 | possible physiological acceptor | Oryctolagus cuniculus |
General Stability | Organism |
---|---|
oxalate stabilizes | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation during preincubation at 0°C; progressive inactivation, substrate decreases rate of inactivation | Oryctolagus cuniculus | |
EDTA | progressive inactivation, substrate decreases rate of inactivation | Oryctolagus cuniculus | |
KCN | competitive, reversible | Oryctolagus cuniculus | |
L-lactate | weak | Oryctolagus cuniculus | |
oxalate | competitive | Oryctolagus cuniculus | |
oxaloacetate | competitive to acceptor | Oryctolagus cuniculus | |
pyruvate | competitive inhibitor to acceptor | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
D-malate | - |
Oryctolagus cuniculus | |
2 | - |
D-lactate | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + acceptor | Oryctolagus cuniculus | enzyme acts on a variety of (R)-2-hydroxy acids | pyruvate + reduced acceptor | - |
? | |
(R)-lactate + acceptor | Oryctolagus cuniculus | cytochrome c, ubiquinone and O2 are possible physiological electron acceptors | pyruvate + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
0°C, as precipitate in ammonium sulfate stable for months | Oryctolagus cuniculus |
4°C, in 25 mM Tris buffer, pH 8.0, stable for weeks | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + acceptor | cytochrome c, ubiquinone and O2 are possible physiological acceptors | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
(R)-lactate + acceptor | no activity with glycolate, 3-methylpentanoate, phenylglycolate, phenyllactate, 3-phosphoglycerate, 6-phosphoglycerate | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
(R)-lactate + acceptor | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
(R)-lactate + acceptor | enzyme acts on a variety of (R)-2-hydroxy acids | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
(R)-lactate + acceptor | cytochrome c, ubiquinone and O2 are possible physiological electron acceptors | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
2(R)-hydroxybutanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxobutanoate + reduced acceptor | - |
? | |
2(R)-hydroxyheptanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxoheptanoate + reduced acceptor | - |
? | |
2(R)-hydroxyhexanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxohexanoate + reduced acceptor | - |
? | |
2(R)-hydroxyoctanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxooctanoate + reduced acceptor | - |
? | |
2(R)-hydroxypentanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxopentanoate + reduced acceptor | - |
? | |
D-glycerate + acceptor | - |
Oryctolagus cuniculus | hydroxypyruvate + reduced acceptor | - |
? | |
D-malate + acceptor | - |
Oryctolagus cuniculus | oxaloacetate + reduced acceptor | - |
? | |
D-tartrate + acceptor | poor substrate | Oryctolagus cuniculus | 2-oxo-3-hydroxybutanoate + reduced acceptor | - |
? | |
meso-tartrate + acceptor | good substrate | Oryctolagus cuniculus | 2-oxo-3-hydroxybutanoate + reduced acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
and below for malate as substrate | Oryctolagus cuniculus |
8.6 | - |
D-lactate as substrate | Oryctolagus cuniculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in 25 mM Tris buffer at 4°C stable for weeks | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | - |
Oryctolagus cuniculus | |
cytochrome c | possible physiological acceptor | Oryctolagus cuniculus | |
FAD | flavoprotein, 2 molecules per molecule of enzyme, not replaceable by FMN or riboflavin | Oryctolagus cuniculus | |
phenazine methosulfate | - |
Oryctolagus cuniculus | |
ubiquinone | possible physiological acceptor | Oryctolagus cuniculus |