BRENDA - Enzyme Database
show all sequences of 1.1.99.6

D-2-hydroxy acid dehydrogenase from animal tissue

Cammack, R.; Methods Enzymol. 41, 323-329 (1975)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ferricyanide
100 mM, 2.2fold increase in enzyme activity
Oryctolagus cuniculus
methylene blue
-
Oryctolagus cuniculus
additional information
NAD+ or NADP+ do not act as acceptors
Oryctolagus cuniculus
O2
possible physiological acceptor
Oryctolagus cuniculus
General Stability
General Stability
Organism
oxalate stabilizes
Oryctolagus cuniculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
inactivation during preincubation at 0°C; progressive inactivation, substrate decreases rate of inactivation
Oryctolagus cuniculus
EDTA
progressive inactivation, substrate decreases rate of inactivation
Oryctolagus cuniculus
KCN
competitive, reversible
Oryctolagus cuniculus
L-lactate
weak
Oryctolagus cuniculus
oxalate
competitive
Oryctolagus cuniculus
oxaloacetate
competitive to acceptor
Oryctolagus cuniculus
pyruvate
competitive inhibitor to acceptor
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
D-malate
-
Oryctolagus cuniculus
2
-
D-lactate
-
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
particle-bound
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
102000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + acceptor
Oryctolagus cuniculus
enzyme acts on a variety of (R)-2-hydroxy acids
pyruvate + reduced acceptor
-
Oryctolagus cuniculus
?
(R)-lactate + acceptor
Oryctolagus cuniculus
cytochrome c, ubiquinone and O2 are possible physiological electron acceptors
pyruvate + reduced acceptor
-
Oryctolagus cuniculus
?
Organism
Organism
UniProt
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.4
-
-
Oryctolagus cuniculus
Storage Stability
Storage Stability
Organism
0°C, as precipitate in ammonium sulfate stable for months
Oryctolagus cuniculus
4°C, in 25 mM Tris buffer, pH 8.0, stable for weeks
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-lactate + acceptor
cytochrome c, ubiquinone and O2 are possible physiological acceptors
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
no activity with glycolate, 3-methylpentanoate, phenylglycolate, phenyllactate, 3-phosphoglycerate, 6-phosphoglycerate
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
enzyme acts on a variety of (R)-2-hydroxy acids
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
cytochrome c, ubiquinone and O2 are possible physiological electron acceptors
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxybutanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxobutanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxyheptanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxoheptanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxyhexanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxohexanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxyoctanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxooctanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxypentanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxopentanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
D-glycerate + acceptor
-
287840
Oryctolagus cuniculus
hydroxypyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
D-malate + acceptor
-
287840
Oryctolagus cuniculus
oxaloacetate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
D-tartrate + acceptor
poor substrate
287840
Oryctolagus cuniculus
2-oxo-3-hydroxybutanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
meso-tartrate + acceptor
good substrate
287840
Oryctolagus cuniculus
2-oxo-3-hydroxybutanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
and below for malate as substrate
Oryctolagus cuniculus
8.6
-
D-lactate as substrate
Oryctolagus cuniculus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
in 25 mM Tris buffer at 4°C stable for weeks
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
-
Oryctolagus cuniculus
cytochrome c
possible physiological acceptor
Oryctolagus cuniculus
FAD
flavoprotein, 2 molecules per molecule of enzyme, not replaceable by FMN or riboflavin
Oryctolagus cuniculus
phenazine methosulfate
-
Oryctolagus cuniculus
ubiquinone
possible physiological acceptor
Oryctolagus cuniculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ferricyanide
100 mM, 2.2fold increase in enzyme activity
Oryctolagus cuniculus
methylene blue
-
Oryctolagus cuniculus
additional information
NAD+ or NADP+ do not act as acceptors
Oryctolagus cuniculus
O2
possible physiological acceptor
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
-
Oryctolagus cuniculus
cytochrome c
possible physiological acceptor
Oryctolagus cuniculus
FAD
flavoprotein, 2 molecules per molecule of enzyme, not replaceable by FMN or riboflavin
Oryctolagus cuniculus
phenazine methosulfate
-
Oryctolagus cuniculus
ubiquinone
possible physiological acceptor
Oryctolagus cuniculus
General Stability (protein specific)
General Stability
Organism
oxalate stabilizes
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
inactivation during preincubation at 0°C; progressive inactivation, substrate decreases rate of inactivation
Oryctolagus cuniculus
EDTA
progressive inactivation, substrate decreases rate of inactivation
Oryctolagus cuniculus
KCN
competitive, reversible
Oryctolagus cuniculus
L-lactate
weak
Oryctolagus cuniculus
oxalate
competitive
Oryctolagus cuniculus
oxaloacetate
competitive to acceptor
Oryctolagus cuniculus
pyruvate
competitive inhibitor to acceptor
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
D-malate
-
Oryctolagus cuniculus
2
-
D-lactate
-
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
particle-bound
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
102000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + acceptor
Oryctolagus cuniculus
enzyme acts on a variety of (R)-2-hydroxy acids
pyruvate + reduced acceptor
-
Oryctolagus cuniculus
?
(R)-lactate + acceptor
Oryctolagus cuniculus
cytochrome c, ubiquinone and O2 are possible physiological electron acceptors
pyruvate + reduced acceptor
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.4
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, as precipitate in ammonium sulfate stable for months
Oryctolagus cuniculus
4°C, in 25 mM Tris buffer, pH 8.0, stable for weeks
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-lactate + acceptor
cytochrome c, ubiquinone and O2 are possible physiological acceptors
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
no activity with glycolate, 3-methylpentanoate, phenylglycolate, phenyllactate, 3-phosphoglycerate, 6-phosphoglycerate
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
enzyme acts on a variety of (R)-2-hydroxy acids
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
(R)-lactate + acceptor
cytochrome c, ubiquinone and O2 are possible physiological electron acceptors
287840
Oryctolagus cuniculus
pyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxybutanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxobutanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxyheptanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxoheptanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxyhexanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxohexanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxyoctanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxooctanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
2(R)-hydroxypentanoate + acceptor
-
287840
Oryctolagus cuniculus
2-oxopentanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
D-glycerate + acceptor
-
287840
Oryctolagus cuniculus
hydroxypyruvate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
D-malate + acceptor
-
287840
Oryctolagus cuniculus
oxaloacetate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
D-tartrate + acceptor
poor substrate
287840
Oryctolagus cuniculus
2-oxo-3-hydroxybutanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
meso-tartrate + acceptor
good substrate
287840
Oryctolagus cuniculus
2-oxo-3-hydroxybutanoate + reduced acceptor
-
287840
Oryctolagus cuniculus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
and below for malate as substrate
Oryctolagus cuniculus
8.6
-
D-lactate as substrate
Oryctolagus cuniculus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
in 25 mM Tris buffer at 4°C stable for weeks
Oryctolagus cuniculus
Other publictions for EC 1.1.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721200
Shibahara
Crystallization and preliminar ...
Aeropyrum pernix, Aeropyrum pernix DSM 11879
Acta Crystallogr. Sect. F
67
1425-1427
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693285
Satomura
A novel flavin adenine dinucle ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
J. Biosci. Bioeng.
106
16-21
2008
-
-
1
-
-
-
5
3
-
-
3
-
-
8
-
-
1
-
-
-
-
-
10
1
2
2
-
2
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
5
-
3
-
-
3
-
-
-
-
1
-
-
-
-
10
1
2
-
2
-
1
1
1
-
-
-
-
-
-
-
721463
Pagala
Cellular localization of D-lac ...
Archaeoglobus fulgidus
Archaea
1
95-104
2002
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287843
Huang
Cloning and characterization o ...
Homo sapiens
Biochem. Biophys. Res. Commun.
268
298-301
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287842
Hekmat
-
Production of pyruvate from (R ...
Proteus vulgaris
Enzyme Microb. Technol.
24
471-479
1999
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722504
Reed
The Archaeoglobus fulgidus D-l ...
Archaeoglobus fulgidus
J. Bacteriol.
181
7580-7587
1999
4
-
1
-
-
-
2
1
-
1
-
-
-
3
-
-
1
-
-
-
-
-
3
-
2
2
1
1
-
1
1
-
1
-
-
-
4
-
1
1
-
-
-
-
2
-
1
-
1
-
-
-
-
-
1
-
-
-
-
3
-
2
1
1
-
1
1
-
-
-
-
-
-
-
-
287841
Trautwein
The (2R)-hydroxycarboxylate-vi ...
Proteus vulgaris
Eur. J. Biochem.
222
1025-1032
1994
-
-
-
-
-
-
1
-
-
1
2
-
-
1
1
-
1
-
-
-
2
-
5
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
1
2
-
-
1
-
1
-
-
2
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287840
Cammack
D-2-hydroxy acid dehydrogenase ...
Oryctolagus cuniculus
Methods Enzymol.
41
323-329
1975
4
-
-
-
-
1
7
2
1
-
1
2
-
2
-
-
1
-
-
1
1
2
14
-
-
1
-
-
-
2
-
1
5
-
-
-
4
-
-
5
-
-
1
-
7
-
2
1
-
1
2
-
-
-
1
-
1
1
2
14
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
287839
Tubbs
Dehydrogenation of D-lactate b ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
34
290-291
1959
4
-
-
-
-
-
6
1
1
1
-
1
-
1
-
-
1
-
-
2
-
-
2
-
-
2
-
-
-
1
-
-
3
-
-
-
4
-
-
3
-
-
-
-
6
-
1
1
1
-
1
-
-
-
1
-
2
-
-
2
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-