Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | 5 mM, 50% inhibition after 7 min, complete inhibition after 1 h | Proteus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | molybdenum containing enzyme | Proteus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, probably octamer, SDS-PAGE | Proteus vulgaris |
180000 | 600000 | gel filtration, native PAGE | Proteus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h | Proteus vulgaris |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, hydroxyapatite, gel filtration | Proteus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
270 | - |
80000 Da form of the enzyme | Proteus vulgaris |
1800 | - |
600000 Da form of the enzyme | Proteus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + acceptor | - |
Proteus vulgaris | pyruvate + reduced acceptor | - |
r | |
D-gluconate + acceptor | - |
Proteus vulgaris | 2-oxo-gluconate + reduced acceptor | - |
r | |
D-glucose 6-phosphate + acceptor | - |
Proteus vulgaris | ? | - |
r | |
N-acetylneuraminate + acceptor | - |
Proteus vulgaris | ? | - |
r | |
phenylpyruvate + reduced acceptor | - |
Proteus vulgaris | phenyl-(R)-lactate + acceptor | - |
r |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 80000, probably octamer, SDS-PAGE | Proteus vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
anthraquinone-2,6-disulfonate | artificial electron acceptor | Proteus vulgaris | |
molybdopterin mononucleotide | - |
Proteus vulgaris | |
additional information | molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit | Proteus vulgaris |