BRENDA - Enzyme Database show
show all sequences of 1.1.99.6

The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein

Trautwein, T.; Krauss, F.; Lottspeich, F.; Simon, H.; Eur. J. Biochem. 222, 1025-1032 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
KCN
5 mM, 50% inhibition after 7 min, complete inhibition after 1 h
Proteus vulgaris
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Molybdenum
molybdenum containing enzyme
Proteus vulgaris
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
180000
600000
gel filtration, native PAGE
Proteus vulgaris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Proteus vulgaris
-
-
-
Oxidation Stability
Oxidation Stability
Organism
oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h
Proteus vulgaris
Purification (Commentary)
Commentary
Organism
DEAE-cellulose, hydroxyapatite, gel filtration
Proteus vulgaris
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
270
-
80000 Da form of the enzyme
Proteus vulgaris
1800
-
600000 Da form of the enzyme
Proteus vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-lactate + acceptor
-
287841
Proteus vulgaris
pyruvate + reduced acceptor
-
287841
Proteus vulgaris
r
D-gluconate + acceptor
-
287841
Proteus vulgaris
2-oxo-gluconate + reduced acceptor
-
287841
Proteus vulgaris
r
D-glucose 6-phosphate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
N-acetylneuraminate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
phenylpyruvate + reduced acceptor
-
287841
Proteus vulgaris
phenyl-(R)-lactate + acceptor
-
287841
Proteus vulgaris
r
Subunits
Subunits
Commentary
Organism
oligomer
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
anthraquinone-2,6-disulfonate
artificial electron acceptor
Proteus vulgaris
molybdopterin mononucleotide
-
Proteus vulgaris
additional information
molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit
Proteus vulgaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
anthraquinone-2,6-disulfonate
artificial electron acceptor
Proteus vulgaris
molybdopterin mononucleotide
-
Proteus vulgaris
additional information
molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit
Proteus vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCN
5 mM, 50% inhibition after 7 min, complete inhibition after 1 h
Proteus vulgaris
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Molybdenum
molybdenum containing enzyme
Proteus vulgaris
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
180000
600000
gel filtration, native PAGE
Proteus vulgaris
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h
Proteus vulgaris
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-cellulose, hydroxyapatite, gel filtration
Proteus vulgaris
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
270
-
80000 Da form of the enzyme
Proteus vulgaris
1800
-
600000 Da form of the enzyme
Proteus vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-lactate + acceptor
-
287841
Proteus vulgaris
pyruvate + reduced acceptor
-
287841
Proteus vulgaris
r
D-gluconate + acceptor
-
287841
Proteus vulgaris
2-oxo-gluconate + reduced acceptor
-
287841
Proteus vulgaris
r
D-glucose 6-phosphate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
N-acetylneuraminate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
phenylpyruvate + reduced acceptor
-
287841
Proteus vulgaris
phenyl-(R)-lactate + acceptor
-
287841
Proteus vulgaris
r
Subunits (protein specific)
Subunits
Commentary
Organism
oligomer
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
Other publictions for EC 1.1.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721200
Shibahara
Crystallization and preliminar ...
Aeropyrum pernix, Aeropyrum pernix DSM 11879
Acta Crystallogr. Sect. F
67
1425-1427
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693285
Satomura
A novel flavin adenine dinucle ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
J. Biosci. Bioeng.
106
16-21
2008
-
-
1
-
-
-
5
3
-
-
3
-
-
8
-
-
1
-
-
-
-
-
10
1
2
-
2
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
5
-
3
-
-
3
-
-
-
-
1
-
-
-
-
10
1
2
-
2
-
1
1
1
-
-
-
-
-
-
-
721463
Pagala
Cellular localization of D-lac ...
Archaeoglobus fulgidus
Archaea
1
95-104
2002
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287843
Huang
Cloning and characterization o ...
Homo sapiens
Biochem. Biophys. Res. Commun.
268
298-301
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287842
Hekmat
-
Production of pyruvate from (R ...
Proteus vulgaris
Enzyme Microb. Technol.
24
471-479
1999
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722504
Reed
The Archaeoglobus fulgidus D-l ...
Archaeoglobus fulgidus
J. Bacteriol.
181
7580-7587
1999
4
-
1
-
-
-
2
1
-
1
-
-
-
3
-
-
1
-
-
-
-
-
3
-
2
1
1
-
1
1
-
1
-
-
-
4
-
1
1
-
-
-
-
2
-
1
-
1
-
-
-
-
-
1
-
-
-
-
3
-
2
1
1
-
1
1
-
-
-
-
-
-
-
-
287841
Trautwein
The (2R)-hydroxycarboxylate-vi ...
Proteus vulgaris
Eur. J. Biochem.
222
1025-1032
1994
-
-
-
-
-
-
1
-
-
1
2
-
-
1
1
-
1
-
-
-
2
-
5
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
1
2
-
-
1
-
1
-
-
2
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287840
Cammack
D-2-hydroxy acid dehydrogenase ...
Oryctolagus cuniculus
Methods Enzymol.
41
323-329
1975
4
-
-
-
-
1
7
2
1
-
1
2
-
2
-
-
1
-
-
1
1
2
14
-
1
-
-
-
2
-
1
5
-
-
-
4
-
-
5
-
-
1
-
7
-
2
1
-
1
2
-
-
-
1
-
1
1
2
14
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
287839
Tubbs
Dehydrogenation of D-lactate b ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
34
290-291
1959
4
-
-
-
-
-
6
1
1
1
-
1
-
1
-
-
1
-
-
2
-
-
2
-
2
-
-
-
1
-
-
3
-
-
-
4
-
-
3
-
-
-
-
6
-
1
1
1
-
1
-
-
-
1
-
2
-
-
2
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-