show 
Purification and characterization of a carbohydrate: acceptor oxidoreductase from Paraconiothyrium sp. that produces lactobionic acid efficiently
Kiryu, T.; Nakano, H.; Kiso, T.; Murakami, H.; Biosci. Biotechnol. Biochem. 72, 833-841 (2008)
Data extracted from this reference:
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
Cellopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.06
-
cellohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.06
-
Cellotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.06
-
cellotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.08
-
cellobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.09
-
xylobiose
O2 used as electron acceptor
Paraconiothyrium sp.
0.11
-
cellotriose
O2 used as electron acceptor
Paraconiothyrium sp.
0.11
-
lactose
O2 used as electron acceptor
Paraconiothyrium sp.
0.13
-
cellobiose
O2 used as electron acceptor
Paraconiothyrium sp.
0.15
-
Cellopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
0.16
-
cellohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
0.19
-
lactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.21
-
xylobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.26
-
Cellotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
2
3
D-xylose
O2 used as electron acceptor
Paraconiothyrium sp.
4.9
-
maltose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
6.7
-
D-xylose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
11
-
maltopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
11
-
maltose
O2 used as electron acceptor
Paraconiothyrium sp.
12
-
maltotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
13
-
maltohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
18
-
maltotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
20
-
D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
25
-
D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
30
-
maltohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
30
-
maltotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
30
-
maltotriose
O2 used as electron acceptor
Paraconiothyrium sp.
32
-
maltopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
47
-
L-arabinose
O2 used as electron acceptor
Paraconiothyrium sp.
52
-
6-deoxy-D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
61
-
D-galactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
75
-
6-deoxy-D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
85
-
L-arabinose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
91
-
D-galactose
O2 used as electron acceptor
Paraconiothyrium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
gel filtration, SDS-PAGE
Paraconiothyrium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paraconiothyrium sp.
-
isolated as fungal strain KD-3 from soil, identified as Paraconiothyrium sp. according to 18S rDNA and ITS-5.8 S rDNA analysis
-
Purification (Commentary)
Commentary
Organism
gel filtration, SDS-PAGE
Paraconiothyrium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
enzyme purified and characterized, identified as a carbohydrate-acceptor oxidoreductase according to its molecular and structural properties and according to its specificity for electron donors and acceptors
Paraconiothyrium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-deoxy-D-glucose + 2,6-dichlorophenol indophenol
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
6-deoxy-D-glucose + O2
hexoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
beta-D-glucose + 2,6-dichlorophenol indophenol
DCIP, electron acceptor specificity determined
685689
Paraconiothyrium sp.
D-glucono-1,5-lactone + ?
-
-
-
?
beta-D-glucose + O2
kinetic parameters for various sugars shown, enzyme properties compared to other sugar oxidizing enzymes
685689
Paraconiothyrium sp.
D-glucono-1,5-lactone + H2O2
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
cellobiose + O2
disaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellohexaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellopentaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellotetraose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellotriose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-fructose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-galactose + 2,6-dichlorophenol indophenol
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
D-galactose + O2
hexoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-glucose + acceptor
-
685689
Paraconiothyrium sp.
?
-
-
-
-
D-mannose + O2
hexoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-xylobiose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-xylose + 2,6-dichlorophenol indophenol
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
D-xylose + O2
pentoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
L-arabinose + 2,6-dichlorophenol indophenol
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
L-arabinose + O2
pentoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
substrate specificity for alpha-lactose and beta-lactose determined
685689
Paraconiothyrium sp.
?
-
-
-
?
lactose + O2
substrate specificity for alpha-lactose and beta-lactose determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltohexaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltopentaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltose + 2,6-dichlorophenol indophenol
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
maltose + O2
disaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltotetraose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltotriose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
xylobiose + 2,6-dichlorophenol indophenol
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
xylobiose + O2
disaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
D-galactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.35
-
maltohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.45
-
6-deoxy-D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
0.46
-
D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.57
-
maltohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
0.58
-
D-xylose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.73
-
L-arabinose
O2 used as electron acceptor
Paraconiothyrium sp.
0.76
-
maltose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.81
-
6-deoxy-D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.9
-
maltopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.1
-
cellohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.1
-
Cellopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.1
-
L-arabinose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.4
-
Cellotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.5
-
cellotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.5
-
D-galactose
O2 used as electron acceptor
Paraconiothyrium sp.
1.7
-
D-xylose
O2 used as electron acceptor
Paraconiothyrium sp.
1.8
-
maltopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
2
-
maltose
O2 used as electron acceptor
Paraconiothyrium sp.
2.1
-
D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
2.2
-
maltotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
2.3
-
maltotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
2.3
-
maltotriose
O2 used as electron acceptor
Paraconiothyrium sp.
2.6
-
maltotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
3
-
xylobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
3.3
-
xylobiose
O2 used as electron acceptor
Paraconiothyrium sp.
4.4
-
lactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
6.3
-
lactose
O2 used as electron acceptor
Paraconiothyrium sp.
7.4
-
cellobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
7.5
-
cellotriose
O2 used as electron acceptor
Paraconiothyrium sp.
9
-
cellobiose
O2 used as electron acceptor
Paraconiothyrium sp.
9
-
Cellopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
10
-
cellohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
11
-
Cellotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
most active at pH 5.5 if O2 is used as electron acceptor
Paraconiothyrium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
2
7
stable in a pH range of 2.0-7.0 at 30°C
Paraconiothyrium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
absorption spectra determined
Paraconiothyrium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
absorption spectra determined
Paraconiothyrium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
Cellopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.06
-
cellohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.06
-
Cellotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.06
-
cellotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.08
-
cellobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.09
-
xylobiose
O2 used as electron acceptor
Paraconiothyrium sp.
0.11
-
cellotriose
O2 used as electron acceptor
Paraconiothyrium sp.
0.11
-
lactose
O2 used as electron acceptor
Paraconiothyrium sp.
0.13
-
cellobiose
O2 used as electron acceptor
Paraconiothyrium sp.
0.15
-
Cellopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
0.16
-
cellohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
0.19
-
lactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.21
-
xylobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.26
-
Cellotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
2
3
D-xylose
O2 used as electron acceptor
Paraconiothyrium sp.
4.9
-
maltose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
6.7
-
D-xylose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
11
-
maltopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
11
-
maltose
O2 used as electron acceptor
Paraconiothyrium sp.
12
-
maltotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
13
-
maltohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
18
-
maltotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
20
-
D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
25
-
D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
30
-
maltohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
30
-
maltotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
30
-
maltotriose
O2 used as electron acceptor
Paraconiothyrium sp.
32
-
maltopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
47
-
L-arabinose
O2 used as electron acceptor
Paraconiothyrium sp.
52
-
6-deoxy-D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
61
-
D-galactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
75
-
6-deoxy-D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
85
-
L-arabinose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
91
-
D-galactose
O2 used as electron acceptor
Paraconiothyrium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
gel filtration, SDS-PAGE
Paraconiothyrium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration, SDS-PAGE
Paraconiothyrium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
enzyme purified and characterized, identified as a carbohydrate-acceptor oxidoreductase according to its molecular and structural properties and according to its specificity for electron donors and acceptors
Paraconiothyrium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-deoxy-D-glucose + 2,6-dichlorophenol indophenol
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
6-deoxy-D-glucose + O2
hexoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
beta-D-glucose + 2,6-dichlorophenol indophenol
DCIP, electron acceptor specificity determined
685689
Paraconiothyrium sp.
D-glucono-1,5-lactone + ?
-
-
-
?
beta-D-glucose + O2
kinetic parameters for various sugars shown, enzyme properties compared to other sugar oxidizing enzymes
685689
Paraconiothyrium sp.
D-glucono-1,5-lactone + H2O2
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
cellobiose + O2
disaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellohexaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellopentaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellotetraose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
cellotriose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-fructose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-galactose + 2,6-dichlorophenol indophenol
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
D-galactose + O2
hexoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-glucose + acceptor
-
685689
Paraconiothyrium sp.
?
-
-
-
-
D-mannose + O2
hexoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-xylobiose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
D-xylose + 2,6-dichlorophenol indophenol
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
D-xylose + O2
pentoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
L-arabinose + 2,6-dichlorophenol indophenol
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
L-arabinose + O2
pentoses as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
substrate specificity for alpha-lactose and beta-lactose determined
685689
Paraconiothyrium sp.
?
-
-
-
?
lactose + O2
substrate specificity for alpha-lactose and beta-lactose determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltohexaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltopentaose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltose + 2,6-dichlorophenol indophenol
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
maltose + O2
disaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltotetraose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
maltotriose + O2
oligosaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
xylobiose + 2,6-dichlorophenol indophenol
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
685689
Paraconiothyrium sp.
?
-
-
-
?
xylobiose + O2
disaccharides as substrates determined
685689
Paraconiothyrium sp.
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
D-galactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.35
-
maltohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.45
-
6-deoxy-D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
0.46
-
D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.57
-
maltohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
0.58
-
D-xylose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.73
-
L-arabinose
O2 used as electron acceptor
Paraconiothyrium sp.
0.76
-
maltose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.81
-
6-deoxy-D-glucose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
0.9
-
maltopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.1
-
cellohexaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.1
-
Cellopentaose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.1
-
L-arabinose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.4
-
Cellotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.5
-
cellotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
1.5
-
D-galactose
O2 used as electron acceptor
Paraconiothyrium sp.
1.7
-
D-xylose
O2 used as electron acceptor
Paraconiothyrium sp.
1.8
-
maltopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
2
-
maltose
O2 used as electron acceptor
Paraconiothyrium sp.
2.1
-
D-glucose
O2 used as electron acceptor
Paraconiothyrium sp.
2.2
-
maltotriose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
2.3
-
maltotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
2.3
-
maltotriose
O2 used as electron acceptor
Paraconiothyrium sp.
2.6
-
maltotetraose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
3
-
xylobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
3.3
-
xylobiose
O2 used as electron acceptor
Paraconiothyrium sp.
4.4
-
lactose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
6.3
-
lactose
O2 used as electron acceptor
Paraconiothyrium sp.
7.4
-
cellobiose
2,6-dichlorophenol indophenol used as electron acceptor
Paraconiothyrium sp.
7.5
-
cellotriose
O2 used as electron acceptor
Paraconiothyrium sp.
9
-
cellobiose
O2 used as electron acceptor
Paraconiothyrium sp.
9
-
Cellopentaose
O2 used as electron acceptor
Paraconiothyrium sp.
10
-
cellohexaose
O2 used as electron acceptor
Paraconiothyrium sp.
11
-
Cellotetraose
O2 used as electron acceptor
Paraconiothyrium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
most active at pH 5.5 if O2 is used as electron acceptor
Paraconiothyrium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
2
7
stable in a pH range of 2.0-7.0 at 30°C
Paraconiothyrium sp.
Other publictions for EC 1.1.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743824
Vuong
Direct comparison of gluco-ol ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
15
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
-
-
2
2
-
8
8
741237
Foumani
Enhanced polysaccharide bindin ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
10
e0125398
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
741305
Vuong
Fusion of a xylan-binding modu ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
9
e95170
2014
-
-
1
-
-
1
-
-
-
-
1
-
-
8
-
1
1
-
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740204
Vuong
Xylo- and cello-oligosaccharid ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Biofuels
6
148
2013
-
-
-
-
10
-
-
35
-
-
-
-
-
5
-
1
-
-
-
-
-
-
20
-
-
-
-
41
-
-
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
35
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
41
-
-
-
-
-
-
-
-
41
41
724616
Foumani
Altered substrate specificity ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Bioeng.
108
2261-2269
2011
-
-
1
-
3
-
-
36
-
-
-
15
-
4
-
-
1
-
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
36
-
-
-
15
-
-
-
1
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
36
36
685689
Kiryu
Purification and characterizat ...
Paraconiothyrium sp.
Biosci. Biotechnol. Biochem.
72
833-841
2008
-
-
-
-
-
-
-
34
-
-
1
-
-
3
-
-
1
-
-
-
1
-
30
-
-
-
-
34
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
34
-
-
1
-
-
-
-
1
-
-
1
-
30
-
-
-
-
34
1
1
-
-
-
-
-
-
-
-
698829
Huang
Functional roles of the 6-S-cy ...
Sarocladium strictum
J. Biol. Chem.
283
30990-30996
2008
-
-
1
1
4
4
-
3
-
-
-
-
-
2
-
1
1
-
-
-
-
-
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
4
4
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-