BRENDA - Enzyme Database show
show all sequences of 1.1.99.B3

Functional roles of the 6-S-cysteinyl, 8alpha-N1-histidyl FAD in glucooligosaccharide oxidase from Acremonium strictum

Huang, C.H.; Winkler, A.; Chen, C.L.; Lai, W.L.; Tsai, Y.C.; Macheroux, P.; Liaw, S.H.; J. Biol. Chem. 283, 30990-30996 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Sarocladium strictum
Crystallization (Commentary)
Crystallization
Organism
-
Sarocladium strictum
Engineering
Amino acid exchange
Commentary
Organism
C130A
FAD binding site, FAD covalently attached
Sarocladium strictum
H70A
FAD binding site, FAD covalently attached
Sarocladium strictum
H70A/C130A
no activity, lack of essential FAD cofactor
Sarocladium strictum
Y310A
larger amount of carbohydrates
Sarocladium strictum
General Stability
General Stability
Organism
and wild-type proteins are 1.9 M, 2.4 M, 2.6 M, and 3.2 M, respectively.
Sarocladium strictum
and wild-type proteins are 48°C, 52°C, 46°C, and 56°C, respectively.
Sarocladium strictum
The melting temperatures for the H70A, C130A, H70A/C130A,
Sarocladium strictum
The [guanidine HCl]1/2 values for the H70A, C130A, H70A/C130A,
Sarocladium strictum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
cellobiose
wild type protein
Sarocladium strictum
0.22
-
cellobiose
H70A mutant protein
Sarocladium strictum
0.4
-
cellobiose
C130A mutant protein
Sarocladium strictum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sarocladium strictum
Q6PW77
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sarocladium strictum
Purification (Commentary)
Commentary
Organism
-
Sarocladium strictum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cello-oligosaccharide + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
cellobiose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
D-glucose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
lactose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
malto-oligosaccharide + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
maltose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.22
-
cellobiose
H70A mutant protein
Sarocladium strictum
0.58
-
cellobiose
C130A mutant protein
Sarocladium strictum
11.9
-
cellobiose
wild type protein
Sarocladium strictum
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
covalently bound
Sarocladium strictum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Sarocladium strictum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
covalently bound
Sarocladium strictum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Sarocladium strictum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C130A
FAD binding site, FAD covalently attached
Sarocladium strictum
H70A
FAD binding site, FAD covalently attached
Sarocladium strictum
H70A/C130A
no activity, lack of essential FAD cofactor
Sarocladium strictum
Y310A
larger amount of carbohydrates
Sarocladium strictum
General Stability (protein specific)
General Stability
Organism
and wild-type proteins are 1.9 M, 2.4 M, 2.6 M, and 3.2 M, respectively.
Sarocladium strictum
and wild-type proteins are 48°C, 52°C, 46°C, and 56°C, respectively.
Sarocladium strictum
The melting temperatures for the H70A, C130A, H70A/C130A,
Sarocladium strictum
The [guanidine HCl]1/2 values for the H70A, C130A, H70A/C130A,
Sarocladium strictum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
cellobiose
wild type protein
Sarocladium strictum
0.22
-
cellobiose
H70A mutant protein
Sarocladium strictum
0.4
-
cellobiose
C130A mutant protein
Sarocladium strictum
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sarocladium strictum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sarocladium strictum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cello-oligosaccharide + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
cellobiose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
D-glucose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
lactose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
malto-oligosaccharide + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
maltose + acceptor
-
698829
Sarocladium strictum
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.22
-
cellobiose
H70A mutant protein
Sarocladium strictum
0.58
-
cellobiose
C130A mutant protein
Sarocladium strictum
11.9
-
cellobiose
wild type protein
Sarocladium strictum
Other publictions for EC 1.1.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743824
Vuong
Direct comparison of gluco-ol ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
15
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
-
-
2
2
-
8
8
741237
Foumani
Enhanced polysaccharide bindin ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
10
e0125398
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
741305
Vuong
Fusion of a xylan-binding modu ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
9
e95170
2014
-
-
1
-
-
1
-
-
-
-
1
-
-
8
-
1
1
-
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740204
Vuong
Xylo- and cello-oligosaccharid ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Biofuels
6
148
2013
-
-
-
-
10
-
-
35
-
-
-
-
-
5
-
1
-
-
-
-
-
-
20
-
-
-
-
41
-
-
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
35
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
41
-
-
-
-
-
-
-
-
41
41
724616
Foumani
Altered substrate specificity ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Bioeng.
108
2261-2269
2011
-
-
1
-
3
-
-
36
-
-
-
15
-
4
-
-
1
-
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
36
-
-
-
15
-
-
-
1
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
36
36
685689
Kiryu
Purification and characterizat ...
Paraconiothyrium sp.
Biosci. Biotechnol. Biochem.
72
833-841
2008
-
-
-
-
-
-
-
34
-
-
1
-
-
3
-
-
1
-
-
-
1
-
30
-
-
-
-
34
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
34
-
-
1
-
-
-
-
1
-
-
1
-
30
-
-
-
-
34
1
1
-
-
-
-
-
-
-
-
698829
Huang
Functional roles of the 6-S-cy ...
Sarocladium strictum
J. Biol. Chem.
283
30990-30996
2008
-
-
1
1
4
4
-
3
-
-
-
-
-
2
-
1
1
-
-
-
-
-
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
4
4
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-