BRENDA - Enzyme Database show
show all sequences of 1.1.99.B3

Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum

Foumani, M.; Vuong, T.V.; Master, E.R.; Biotechnol. Bioeng. 108, 2261-2269 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris strain GS115
Sarocladium strictum
Engineering
Amino acid exchange
Commentary
Organism
W351F
the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates
Sarocladium strictum
Y300A
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
Sarocladium strictum
Y300N
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
Sarocladium strictum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
cellobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.083
-
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.085
-
cellotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.098
-
xylobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.1
-
xylotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.11
-
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.25
-
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.25
-
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.31
-
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.35
-
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.38
-
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.44
-
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
2.81
-
maltose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
3.11
-
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
3.15
-
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
4.3
-
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
4.83
-
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
5
-
maltose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.11
-
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
8.1
-
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
11
-
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
17.4
-
D-glucose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
19.6
-
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
31
-
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
32
-
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
36.1
-
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
51.8
-
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
56
-
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
92
-
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
96
-
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
105
-
D-xylose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
110
-
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
132
-
D-galactose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
288
-
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
340
-
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
950
-
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cellobiose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
cellobiose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
cellotriose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
cellotriose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
D-galactose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
D-glucose + O2
Sarocladium strictum
highest catalytic efficiency with D-glucose
? + H2O2
-
-
?
D-xylose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
D-xylose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
maltose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
maltose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
additional information
Sarocladium strictum
no activity with mannose
?
-
-
-
additional information
Sarocladium strictum CBS 346.70
no activity with mannose
?
-
-
-
N-acetyl-D-glucosamine + O2
Sarocladium strictum
-
? + H2O2
-
-
?
xylobiose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
xylotriose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sarocladium strictum
Q6PW77
-
-
Sarocladium strictum CBS 346.70
Q6PW77
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA resin column chromatography
Sarocladium strictum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:wheat bran-grown cell
-
Sarocladium strictum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cellobiose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
cellobiose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
cellotriose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
cellotriose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
D-galactose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
D-glucose + O2
highest catalytic efficiency with D-glucose
724616
Sarocladium strictum
? + H2O2
-
-
-
?
D-xylose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
D-xylose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
maltose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
maltose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
additional information
no activity with mannose
724616
Sarocladium strictum
?
-
-
-
-
additional information
no activity with mannose
724616
Sarocladium strictum CBS 346.70
?
-
-
-
-
N-acetyl-D-glucosamine + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
xylobiose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
xylotriose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.6
-
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.3
-
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.3
-
D-xylose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
5.4
-
maltose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.6
-
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.7
-
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
6
-
cellotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
6
-
maltose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
6.3
-
cellobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
6.6
-
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
7.2
-
D-galactose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
7.5
-
D-glucose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
7.8
-
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
7.9
-
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
8
-
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
8.1
-
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
8.3
-
xylotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
8.8
-
xylobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
10
-
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
10.4
-
maltose
mutant enzyme Y300A, at 37°C and pH 8.0; mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
10.8
-
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.2
-
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
11.3
-
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
11.3
-
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.4
-
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.7
-
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.8
-
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
12
-
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
12
-
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
12.8
-
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.2
-
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.3
-
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.3
-
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.7
-
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.9
-
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris strain GS115
Sarocladium strictum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
W351F
the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates
Sarocladium strictum
Y300A
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
Sarocladium strictum
Y300N
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
Sarocladium strictum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
cellobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.083
-
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.085
-
cellotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.098
-
xylobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.1
-
xylotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.11
-
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.25
-
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.25
-
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.31
-
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.35
-
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.38
-
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.44
-
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
2.81
-
maltose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
3.11
-
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
3.15
-
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
4.3
-
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
4.83
-
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
5
-
maltose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.11
-
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
8.1
-
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
11
-
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
17.4
-
D-glucose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
19.6
-
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
31
-
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
32
-
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
36.1
-
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
51.8
-
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
56
-
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
92
-
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
96
-
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
105
-
D-xylose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
110
-
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
132
-
D-galactose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
288
-
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
340
-
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
950
-
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cellobiose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
cellobiose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
cellotriose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
cellotriose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
D-galactose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
D-glucose + O2
Sarocladium strictum
highest catalytic efficiency with D-glucose
? + H2O2
-
-
?
D-xylose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
D-xylose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
maltose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
maltose + O2
Sarocladium strictum CBS 346.70
-
? + H2O2
-
-
?
additional information
Sarocladium strictum
no activity with mannose
?
-
-
-
additional information
Sarocladium strictum CBS 346.70
no activity with mannose
?
-
-
-
N-acetyl-D-glucosamine + O2
Sarocladium strictum
-
? + H2O2
-
-
?
xylobiose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
xylotriose + O2
Sarocladium strictum
-
? + H2O2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography
Sarocladium strictum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:wheat bran-grown cell
-
Sarocladium strictum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cellobiose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
cellobiose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
cellotriose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
cellotriose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
D-galactose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
D-glucose + O2
highest catalytic efficiency with D-glucose
724616
Sarocladium strictum
? + H2O2
-
-
-
?
D-xylose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
D-xylose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
maltose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
maltose + O2
-
724616
Sarocladium strictum CBS 346.70
? + H2O2
-
-
-
?
additional information
no activity with mannose
724616
Sarocladium strictum
?
-
-
-
-
additional information
no activity with mannose
724616
Sarocladium strictum CBS 346.70
?
-
-
-
-
N-acetyl-D-glucosamine + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
xylobiose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
xylotriose + O2
-
724616
Sarocladium strictum
? + H2O2
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.6
-
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.3
-
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.3
-
D-xylose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
5.4
-
maltose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.6
-
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
5.7
-
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
6
-
cellotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
6
-
maltose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
6.3
-
cellobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
6.6
-
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
7.2
-
D-galactose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
7.5
-
D-glucose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
7.8
-
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
7.9
-
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
8
-
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
8.1
-
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
8.3
-
xylotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
8.8
-
xylobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
10
-
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
10.4
-
maltose
mutant enzyme Y300A, at 37°C and pH 8.0; mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
10.8
-
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.2
-
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
11.3
-
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
11.3
-
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.4
-
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.7
-
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
11.8
-
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
12
-
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
12
-
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
12.8
-
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.2
-
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.3
-
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.3
-
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.7
-
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
13.9
-
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.008
-
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.02
-
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.02
-
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.05
-
D-xylose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.06
-
D-galactose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.11
-
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.14
-
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.14
-
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.18
-
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.18
-
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.2
-
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.22
-
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.36
-
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.43
-
D-glucose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.53
-
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.95
-
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
1.1
-
maltose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
1.63
-
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
2.13
-
maltose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
2.48
-
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
2.6
-
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
2.83
-
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
3.6
-
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
4.33
-
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
23.3
-
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
23.3
-
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
25
-
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
30
-
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
45
-
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
48.3
-
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
56.7
-
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
68.3
-
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
70
-
cellotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
85
-
xylotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
90
-
xylobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
100
-
cellobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.008
-
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.02
-
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.02
-
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.05
-
D-xylose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.06
-
D-galactose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.11
-
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.14
-
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.14
-
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.18
-
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.18
-
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
0.2
-
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.22
-
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
0.36
-
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.43
-
D-glucose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
0.53
-
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
0.95
-
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
1.1
-
maltose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
1.63
-
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
2.13
-
maltose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
2.48
-
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
2.6
-
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
2.83
-
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
3.6
-
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
4.33
-
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
23.3
-
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
23.3
-
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
25
-
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
30
-
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
Sarocladium strictum
45
-
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
48.3
-
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
56.7
-
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
Sarocladium strictum
68.3
-
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
Sarocladium strictum
70
-
cellotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
85
-
xylotriose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
90
-
xylobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
100
-
cellobiose
wild type enzyme, at 37°C and pH 8.0
Sarocladium strictum
Other publictions for EC 1.1.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743824
Vuong
Direct comparison of gluco-ol ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
15
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
-
-
2
2
-
8
8
741237
Foumani
Enhanced polysaccharide bindin ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
10
e0125398
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
741305
Vuong
Fusion of a xylan-binding modu ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
9
e95170
2014
-
-
1
-
-
1
-
-
-
-
1
-
-
8
-
1
1
-
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740204
Vuong
Xylo- and cello-oligosaccharid ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Biofuels
6
148
2013
-
-
-
-
10
-
-
35
-
-
-
-
-
5
-
1
-
-
-
-
-
-
20
-
-
-
-
41
-
-
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
35
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
41
-
-
-
-
-
-
-
-
41
41
724616
Foumani
Altered substrate specificity ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Bioeng.
108
2261-2269
2011
-
-
1
-
3
-
-
36
-
-
-
15
-
4
-
-
1
-
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
36
-
-
-
15
-
-
-
1
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
36
36
685689
Kiryu
Purification and characterizat ...
Paraconiothyrium sp.
Biosci. Biotechnol. Biochem.
72
833-841
2008
-
-
-
-
-
-
-
34
-
-
1
-
-
3
-
-
1
-
-
-
1
-
30
-
-
-
-
34
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
34
-
-
1
-
-
-
-
1
-
-
1
-
30
-
-
-
-
34
1
1
-
-
-
-
-
-
-
-
698829
Huang
Functional roles of the 6-S-cy ...
Sarocladium strictum
J. Biol. Chem.
283
30990-30996
2008
-
-
1
1
4
4
-
3
-
-
-
-
-
2
-
1
1
-
-
-
-
-
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
4
4
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-