BRENDA - Enzyme Database show
show all sequences of 1.1.99.B3

Fusion of a xylan-binding module to gluco-oligosaccharide oxidase increases activity and promotes stable immobilization

Vuong, T.V.; Master, E.R.; PLoS ONE 9, e95170 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris strain KM71H
Sarocladium strictum
General Stability
General Stability
Organism
the presence of the xylan-binding module CBM22A of Clostridium thermocellum fused to the N-terminal of the enzyme increases its catalytic activity on mono- and oligo-saccharides by 2-3fold while not affecting binding affinity to these substrates, does not alter the thermostability of the enzyme or reduces substrate inhibition
Sarocladium strictum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
-
Sarocladium strictum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sarocladium strictum
D7PF15
-
-
Sarocladium strictum CBS 346.70
D7PF15
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sarocladium strictum
Purification (Commentary)
Commentary
Organism
Ni-NTA resin column chromatography
Sarocladium strictum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cellobiose + O2
-
741305
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
-
741305
Sarocladium strictum CBS 346.70
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
-
741305
Sarocladium strictum
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
-
741305
Sarocladium strictum CBS 346.70
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
-
741305
Sarocladium strictum
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
-
741305
Sarocladium strictum CBS 346.70
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
741305
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
741305
Sarocladium strictum CBS 346.70
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
741305
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
741305
Sarocladium strictum CBS 346.70
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
-
741305
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
-
-
-
?
debranched soluble oat spelt xylan + acceptor
-
741305
Sarocladium strictum
?
-
-
-
?
soluble oat spelt xylan + acceptor
-
741305
Sarocladium strictum
?
-
-
-
?
xylobiose + O2
highest activity
741305
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
-
741305
Sarocladium strictum
xylohexaono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
-
741305
Sarocladium strictum
xylopentaono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
-
741305
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 76000, calculated from amino acid sequence
Sarocladium strictum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris strain KM71H
Sarocladium strictum
General Stability (protein specific)
General Stability
Organism
the presence of the xylan-binding module CBM22A of Clostridium thermocellum fused to the N-terminal of the enzyme increases its catalytic activity on mono- and oligo-saccharides by 2-3fold while not affecting binding affinity to these substrates, does not alter the thermostability of the enzyme or reduces substrate inhibition
Sarocladium strictum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
-
Sarocladium strictum
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sarocladium strictum
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography
Sarocladium strictum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cellobiose + O2
-
741305
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
-
741305
Sarocladium strictum CBS 346.70
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
-
741305
Sarocladium strictum
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
-
741305
Sarocladium strictum CBS 346.70
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
-
741305
Sarocladium strictum
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
-
741305
Sarocladium strictum CBS 346.70
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
741305
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
741305
Sarocladium strictum CBS 346.70
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
741305
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
741305
Sarocladium strictum CBS 346.70
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
-
741305
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
-
-
-
?
debranched soluble oat spelt xylan + acceptor
-
741305
Sarocladium strictum
?
-
-
-
?
soluble oat spelt xylan + acceptor
-
741305
Sarocladium strictum
?
-
-
-
?
xylobiose + O2
highest activity
741305
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
-
741305
Sarocladium strictum
xylohexaono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
-
741305
Sarocladium strictum
xylopentaono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
-
741305
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 76000, calculated from amino acid sequence
Sarocladium strictum
Other publictions for EC 1.1.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743824
Vuong
Direct comparison of gluco-ol ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
15
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
-
-
2
2
-
8
8
741237
Foumani
Enhanced polysaccharide bindin ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
10
e0125398
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
741305
Vuong
Fusion of a xylan-binding modu ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
9
e95170
2014
-
-
1
-
-
1
-
-
-
-
1
-
-
8
-
1
1
-
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740204
Vuong
Xylo- and cello-oligosaccharid ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Biofuels
6
148
2013
-
-
-
-
10
-
-
35
-
-
-
-
-
5
-
1
-
-
-
-
-
-
20
-
-
-
-
41
-
-
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
35
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
41
-
-
-
-
-
-
-
-
41
41
724616
Foumani
Altered substrate specificity ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Bioeng.
108
2261-2269
2011
-
-
1
-
3
-
-
36
-
-
-
15
-
4
-
-
1
-
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
36
-
-
-
15
-
-
-
1
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
36
36
685689
Kiryu
Purification and characterizat ...
Paraconiothyrium sp.
Biosci. Biotechnol. Biochem.
72
833-841
2008
-
-
-
-
-
-
-
34
-
-
1
-
-
3
-
-
1
-
-
-
1
-
30
-
-
-
-
34
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
34
-
-
1
-
-
-
-
1
-
-
1
-
30
-
-
-
-
34
1
1
-
-
-
-
-
-
-
-
698829
Huang
Functional roles of the 6-S-cy ...
Sarocladium strictum
J. Biol. Chem.
283
30990-30996
2008
-
-
1
1
4
4
-
3
-
-
-
-
-
2
-
1
1
-
-
-
-
-
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
4
4
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-