Data extracted from this reference:
Cloned(Commentary)
expressed in Pichia pastoris strain KM71H
Sarocladium strictum
General Stability
the presence of the xylan-binding module CBM22A of Clostridium thermocellum fused to the N-terminal of the enzyme increases its catalytic activity on mono- and oligo-saccharides by 2-3fold while not affecting binding affinity to these substrates, does not alter the thermostability of the enzyme or reduces substrate inhibition
Sarocladium strictum
Molecular Weight [Da]
76000
Sarocladium strictum
Organism
Sarocladium strictum
D7PF15
Sarocladium strictum CBS 346.70
D7PF15
Posttranslational Modification
glycoprotein
Sarocladium strictum
Purification (Commentary)
Ni-NTA resin column chromatography
Sarocladium strictum
Substrates and Products (Substrate)
cellobiose + O2
741305
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
?
cellobiose + O2
741305
Sarocladium strictum CBS 346.70
cellobiono-1,5-lactone + H2O2
?
cellohexaose + O2
741305
Sarocladium strictum
cellohexaono-1,5-lactone + H2O2
?
cellohexaose + O2
741305
Sarocladium strictum CBS 346.70
cellohexaono-1,5-lactone + H2O2
?
cellopentaose + O2
741305
Sarocladium strictum
cellopentaono-1,5-lactone + H2O2
?
cellopentaose + O2
741305
Sarocladium strictum CBS 346.70
cellopentaono-1,5-lactone + H2O2
?
cellotriose + O2
741305
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
?
cellotriose + O2
741305
Sarocladium strictum CBS 346.70
cellotriono-1,5-lactone + H2O2
?
D-glucose + O2
741305
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
?
D-glucose + O2
741305
Sarocladium strictum CBS 346.70
D-glucono-1,5-lactone + H2O2
?
D-xylose + O2
741305
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
?
debranched soluble oat spelt xylan + acceptor
741305
Sarocladium strictum
?
?
soluble oat spelt xylan + acceptor
741305
Sarocladium strictum
?
?
xylobiose + O2
highest activity
741305
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
?
xylohexaose + O2
741305
Sarocladium strictum
xylohexaono-1,5-lactone + H2O2
?
xylopentaose + O2
741305
Sarocladium strictum
xylopentaono-1,5-lactone + H2O2
?
xylotriose + O2
741305
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
?
Subunits
?
x * 76000, calculated from amino acid sequence
Sarocladium strictum
Synonyms
gluco-oligosaccharide oxidase
Sarocladium strictum
GOOX-VN
Sarocladium strictum
Cloned(Commentary) (protein specific)
expressed in Pichia pastoris strain KM71H
Sarocladium strictum
General Stability (protein specific)
the presence of the xylan-binding module CBM22A of Clostridium thermocellum fused to the N-terminal of the enzyme increases its catalytic activity on mono- and oligo-saccharides by 2-3fold while not affecting binding affinity to these substrates, does not alter the thermostability of the enzyme or reduces substrate inhibition
Sarocladium strictum
Molecular Weight [Da] (protein specific)
76000
Sarocladium strictum
Posttranslational Modification (protein specific)
glycoprotein
Sarocladium strictum
Purification (Commentary) (protein specific)
Ni-NTA resin column chromatography
Sarocladium strictum
Substrates and Products (Substrate) (protein specific)
cellobiose + O2
741305
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
?
cellobiose + O2
741305
Sarocladium strictum CBS 346.70
cellobiono-1,5-lactone + H2O2
?
cellohexaose + O2
741305
Sarocladium strictum
cellohexaono-1,5-lactone + H2O2
?
cellohexaose + O2
741305
Sarocladium strictum CBS 346.70
cellohexaono-1,5-lactone + H2O2
?
cellopentaose + O2
741305
Sarocladium strictum
cellopentaono-1,5-lactone + H2O2
?
cellopentaose + O2
741305
Sarocladium strictum CBS 346.70
cellopentaono-1,5-lactone + H2O2
?
cellotriose + O2
741305
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
?
cellotriose + O2
741305
Sarocladium strictum CBS 346.70
cellotriono-1,5-lactone + H2O2
?
D-glucose + O2
741305
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
?
D-glucose + O2
741305
Sarocladium strictum CBS 346.70
D-glucono-1,5-lactone + H2O2
?
D-xylose + O2
741305
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
?
debranched soluble oat spelt xylan + acceptor
741305
Sarocladium strictum
?
?
soluble oat spelt xylan + acceptor
741305
Sarocladium strictum
?
?
xylobiose + O2
highest activity
741305
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
?
xylohexaose + O2
741305
Sarocladium strictum
xylohexaono-1,5-lactone + H2O2
?
xylopentaose + O2
741305
Sarocladium strictum
xylopentaono-1,5-lactone + H2O2
?
xylotriose + O2
741305
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
?
Subunits (protein specific)
?
x * 76000, calculated from amino acid sequence
Sarocladium strictum
Other publictions for EC 1.1.99.B3
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
2
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743824
Vuong
Direct comparison of gluco-ol ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
16
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
2
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
16
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
-
-
2
2
-
8
8
741237
Foumani
Enhanced polysaccharide bindin ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
10
e0125398
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
8
-
-
1
-
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
741305
Vuong
Fusion of a xylan-binding modu ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
9
e95170
2014
-
-
1
-
-
1
-
-
-
-
1
-
-
12
-
1
1
-
-
-
-
-
17
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740204
Vuong
Xylo- and cello-oligosaccharid ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Biofuels
6
148
2013
-
-
-
-
11
-
-
41
-
-
-
-
-
6
-
1
-
-
-
-
-
-
20
-
2
-
-
-
41
-
-
-
1
-
-
-
-
-
-
1
-
11
-
-
-
-
41
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
41
-
-
-
-
-
-
-
-
41
41
724616
Foumani
Altered substrate specificity ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Bioeng.
108
2261-2269
2011
-
-
1
-
3
-
-
36
-
-
-
15
-
7
-
-
1
-
-
1
-
-
15
-
2
-
-
-
36
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
36
-
-
-
15
-
-
-
1
-
1
-
-
15
-
-
-
-
36
-
-
-
-
-
-
-
-
36
36
685689
Kiryu
Purification and characterizat ...
Paraconiothyrium sp.
Biosci. Biotechnol. Biochem.
72
833-841
2008
-
-
-
-
-
-
-
34
-
-
1
-
-
7
-
-
1
-
-
-
1
-
30
-
-
-
-
-
34
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
34
-
-
1
-
-
-
-
1
-
-
1
-
30
-
-
-
-
34
1
1
-
-
-
-
-
-
-
-
698829
Huang
Functional roles of the 6-S-cy ...
Sarocladium strictum
J. Biol. Chem.
283
30990-30996
2008
-
-
1
1
4
4
-
3
-
-
-
-
-
4
-
1
1
-
-
-
-
-
6
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
4
4
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-