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show all sequences of 1.1.99.B3

Direct comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability

Vuong, T.V.; Foumani, M.; MacCormick, B.; Kwan, R.; Master, E.R.; Sci. Rep. 6, 37356 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris KM71H
Sarocladium strictum
Engineering
Amino acid exchange
Commentary
Organism
Y300A
the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme
Sarocladium strictum
Inhibitors
Inhibitors
Commentary
Organism
Structure
H2O2
about 40% inhibition at 200 mM
Sarocladium strictum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
0.25
-
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
3.15
-
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
5.11
-
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
8.1
-
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
51.8
-
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sarocladium strictum
D7PF15
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA resin column chromatography
Sarocladium strictum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arabinoxylan + O2
-
741429
Sarocladium strictum
?
-
-
-
?
beech wood xylan + O2
-
741429
Sarocladium strictum
?
-
-
-
?
cellobiose + O2
-
741429
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
741429
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
741429
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
-
741429
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
-
-
-
?
maltose + O2
-
741429
Sarocladium strictum
maltono-1,5-lactone + H2O2
-
-
-
?
propoxylated wheat bran hemicellulose + O2
-
741429
Sarocladium strictum
?
-
-
-
?
wheat bran hemicellulose + O2
-
741429
Sarocladium strictum
?
-
-
-
?
xylobiose + O2
highest activity
741429
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
-
741429
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.2
-
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
11.3
-
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.2
-
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.3
-
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.7
-
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.9
-
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris KM71H
Sarocladium strictum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Y300A
the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme
Sarocladium strictum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
H2O2
about 40% inhibition at 200 mM
Sarocladium strictum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
0.25
-
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
3.15
-
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
5.11
-
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
8.1
-
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
51.8
-
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography
Sarocladium strictum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arabinoxylan + O2
-
741429
Sarocladium strictum
?
-
-
-
?
beech wood xylan + O2
-
741429
Sarocladium strictum
?
-
-
-
?
cellobiose + O2
-
741429
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
741429
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
741429
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
-
741429
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
-
-
-
?
maltose + O2
-
741429
Sarocladium strictum
maltono-1,5-lactone + H2O2
-
-
-
?
propoxylated wheat bran hemicellulose + O2
-
741429
Sarocladium strictum
?
-
-
-
?
wheat bran hemicellulose + O2
-
741429
Sarocladium strictum
?
-
-
-
?
xylobiose + O2
highest activity
741429
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
-
741429
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.2
-
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
11.3
-
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.2
-
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.3
-
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.7
-
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
13.9
-
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.22
-
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
1.6
-
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
2.6
-
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
4.3
-
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
45
-
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
56.7
-
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.22
-
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
1.6
-
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
2.6
-
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
4.3
-
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
45
-
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
56.7
-
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37C
Sarocladium strictum
Other publictions for EC 1.1.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
743824
Vuong
Direct comparison of gluco-ol ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
15
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
16
1
-
-
-
-
2
2
-
8
8
741237
Foumani
Enhanced polysaccharide bindin ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
10
e0125398
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
741305
Vuong
Fusion of a xylan-binding modu ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
PLoS ONE
9
e95170
2014
-
-
1
-
-
1
-
-
-
-
1
-
-
8
-
1
1
-
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740204
Vuong
Xylo- and cello-oligosaccharid ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Biofuels
6
148
2013
-
-
-
-
10
-
-
35
-
-
-
-
-
5
-
1
-
-
-
-
-
-
20
-
-
-
-
41
-
-
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
35
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
41
-
-
-
-
-
-
-
-
41
41
724616
Foumani
Altered substrate specificity ...
Sarocladium strictum, Sarocladium strictum CBS 346.70
Biotechnol. Bioeng.
108
2261-2269
2011
-
-
1
-
3
-
-
36
-
-
-
15
-
4
-
-
1
-
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
36
-
-
-
15
-
-
-
1
-
1
-
-
15
-
-
-
-
35
-
-
-
-
-
-
-
-
36
36
685689
Kiryu
Purification and characterizat ...
Paraconiothyrium sp.
Biosci. Biotechnol. Biochem.
72
833-841
2008
-
-
-
-
-
-
-
34
-
-
1
-
-
3
-
-
1
-
-
-
1
-
30
-
-
-
-
34
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
34
-
-
1
-
-
-
-
1
-
-
1
-
30
-
-
-
-
34
1
1
-
-
-
-
-
-
-
-
698829
Huang
Functional roles of the 6-S-cy ...
Sarocladium strictum
J. Biol. Chem.
283
30990-30996
2008
-
-
1
1
4
4
-
3
-
-
-
-
-
2
-
1
1
-
-
-
-
-
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
4
4
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-