BRENDA - Enzyme Database show
show all sequences of 1.10.2.1

Unterschiedliche Cytochrom-b5-Formen als Substrate fuer die L-ascorbat:ferricytochrom-b5-oxidoreduktase (EC1.10.2.1) aus Saeugetierlebermikrosomen

Weber, H.; Weis, W.; Schaeg, W.; Staudinger, H.; Hoppe-Seyler's Z. Physiol. Chem. 354, S.1277-1284 (1973)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
cytochrome b5
pig microsome cytochrome-b5, detergent preparation
Oryctolagus cuniculus
0.0014
-
cytochrome b5
rabbit microsome cytochrome-b5, detergent preparation; rat microsome cytochrome-b5, detergent preparation
Oryctolagus cuniculus
0.0016
-
cytochrome b5
rat microsome cytochrome-b5, trypsin preparation
Oryctolagus cuniculus
0.0016
-
cytochrome b5
rat microsome cytochrome-b5, trypsin preparation
Rattus norvegicus
0.0017
-
cytochrome b5
rabbit microsome cytochrome-b5, trypsin preparation
Sus scrofa
0.0018
-
cytochrome b5
rat microsome cytochrome-b5, detergent preparation
Rattus norvegicus
0.0022
-
cytochrome b5
rabbit microsome cytochrome-b5, trypsin preparation
Rattus norvegicus
0.0022
-
cytochrome b5
rabbit microsome cytochrome-b5, detergent preparation
Sus scrofa
0.0025
-
cytochrome b5
pig microsome cytochrome-b5, detergent preparation
Rattus norvegicus
0.0027
-
cytochrome b5
pig microsome cytochrome-b5, trypsin preparation
Oryctolagus cuniculus
0.0028
-
cytochrome b5
pig microsome cytochrome-b5, trypsin preparation
Sus scrofa
0.003
-
cytochrome b5
rabbit microsome cytochrome-b5, detergent preparation
Rattus norvegicus
0.0037
-
cytochrome b5
rat microsome cytochrome-b5, detergent preparation
Sus scrofa
0.0038
-
cytochrome b5
rabbit microsome cytochrome-b5, trypsin preparation
Oryctolagus cuniculus
0.004
-
cytochrome b5
rat microsome cytochrome-b5, trypsin preparation
Sus scrofa
0.0048
-
cytochrome b5
pig microsome cytochrome-b5, detergent preparation
Sus scrofa
0.009
-
cytochrome b5
pig microsome cytochrome-b5, trypsin preparation
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
microsome
-
Sus scrofa
-
-
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-(+)-ascorbate + ferricytochrome b5
Rattus norvegicus
-
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
Gelb-Silber; rabbit
-
Rattus norvegicus
-
rat, Wistar AF/Han
-
Sus scrofa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-(+)-ascorbate + ferricytochrome b5
-
389969
Rattus norvegicus
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389969
Rattus norvegicus
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389969
Sus scrofa
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389969
Oryctolagus cuniculus
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Oryctolagus cuniculus
7.4
-
-
Rattus norvegicus
7.4
-
-
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
cytochrome b5
pig microsome cytochrome-b5, detergent preparation
Oryctolagus cuniculus
0.0014
-
cytochrome b5
rabbit microsome cytochrome-b5, detergent preparation; rat microsome cytochrome-b5, detergent preparation
Oryctolagus cuniculus
0.0016
-
cytochrome b5
rat microsome cytochrome-b5, trypsin preparation
Oryctolagus cuniculus
0.0016
-
cytochrome b5
rat microsome cytochrome-b5, trypsin preparation
Rattus norvegicus
0.0017
-
cytochrome b5
rabbit microsome cytochrome-b5, trypsin preparation
Sus scrofa
0.0018
-
cytochrome b5
rat microsome cytochrome-b5, detergent preparation
Rattus norvegicus
0.0022
-
cytochrome b5
rabbit microsome cytochrome-b5, trypsin preparation
Rattus norvegicus
0.0022
-
cytochrome b5
rabbit microsome cytochrome-b5, detergent preparation
Sus scrofa
0.0025
-
cytochrome b5
pig microsome cytochrome-b5, detergent preparation
Rattus norvegicus
0.0027
-
cytochrome b5
pig microsome cytochrome-b5, trypsin preparation
Oryctolagus cuniculus
0.0028
-
cytochrome b5
pig microsome cytochrome-b5, trypsin preparation
Sus scrofa
0.003
-
cytochrome b5
rabbit microsome cytochrome-b5, detergent preparation
Rattus norvegicus
0.0037
-
cytochrome b5
rat microsome cytochrome-b5, detergent preparation
Sus scrofa
0.0038
-
cytochrome b5
rabbit microsome cytochrome-b5, trypsin preparation
Oryctolagus cuniculus
0.004
-
cytochrome b5
rat microsome cytochrome-b5, trypsin preparation
Sus scrofa
0.0048
-
cytochrome b5
pig microsome cytochrome-b5, detergent preparation
Sus scrofa
0.009
-
cytochrome b5
pig microsome cytochrome-b5, trypsin preparation
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
microsome
-
Sus scrofa
-
-
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-(+)-ascorbate + ferricytochrome b5
Rattus norvegicus
-
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-(+)-ascorbate + ferricytochrome b5
-
389969
Rattus norvegicus
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389969
Rattus norvegicus
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389969
Sus scrofa
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389969
Oryctolagus cuniculus
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Oryctolagus cuniculus
7.4
-
-
Rattus norvegicus
7.4
-
-
Sus scrofa
Other publictions for EC 1.10.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389975
Knoepfel
Characterization of a cytochro ...
Mus musculus, Oryctolagus cuniculus
Biochem. Biophys. Res. Commun.
291
220-225
2002
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389974
Scherer
-
Participation of L-ascorbate:f ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
359
S.1527-1530
1978
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389973
Scherer
Partial purification of L-asco ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
358
S.1499-1503
1977
2
-
-
-
-
1
-
-
2
-
-
1
-
1
-
-
1
-
-
1
1
1
2
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
1
-
1
1
1
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
389972
Wolf
Discrimination between ascorba ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
72
190-194
1976
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389970
Scherer
Trägergebundenes Cytochrom b5 ...
Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
355
S.1350-1354
1974
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389971
Weber
Monodehydro-L(+)-ascorbat redu ...
Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
355
S.595-599
1974
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389969
Weber
Unterschiedliche Cytochrom-b5- ...
Oryctolagus cuniculus, Rattus norvegicus, Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
354
S.1277-1284
1973
-
-
-
-
-
-
-
17
3
-
-
1
-
3
-
-
-
-
-
2
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
17
3
-
-
1
-
-
-
-
-
2
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
389968
Weber
Praeparative Untersuchungen an ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
353
S.1415-1419
1972
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389967
Everling
Kinetische Untersuchungen an e ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
350
S.1485-1492
1969
-
-
-
-
-
-
1
1
1
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-