Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.1 extracted from

  • Wong, T.C.; Luh, B.S.; Whitaker, J.R.
    Isolation and characterization of polyphenol oxidase isozymes of clingstone peach (1971), Plant Physiol., 48, 19-23.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.1

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid 0.066 mM, 26%, 59% and 96% inhibition of isoenzymes B, C, and D, respectively Prunus persica
CN- 0.165 mM, 84%, 70%, 100% and 40% inhibition of isoenzymes A, B, C and D, respectively Prunus persica
diethyldithiocarbamate 0.066 mM, 31%, 40%, 36% and 84% inhibition of isoenzymes A, B, C, and D, respectively Prunus persica
EDTA 100 mM, 33%, 60%, 81% and 35% inhibition of isoenzymes A, B, C and D, respectively Prunus persica
glutathione 0.066 mM, 2%, 22% and 84% inhibition of isoenzymes B, C, and D, respectively Prunus persica
NaCl 800 mM, 48%, 47%, 55% and 93% inhibition of isoenzymes A, B, C, and D, respectively Prunus persica
NaHSO3 0.066 mM, 64%, 50% and 27% inhibition of isoenzymes B, C, and D, respectively Prunus persica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
 catechol isoenzyme B Prunus persica
6.6
-
 catechol isoenzyme A Prunus persica
7
-
 catechol isoenzyme C Prunus persica
36
-
 catechol isoenzyme D Prunus persica

Organism

Organism UniProt Comment Textmining
Prunus persica
-
 peach
-

Purification (Commentary)

Purification (Comment) Organism
isoenzymes A, B, C and D, acetone precipitate, DEAE-cellulose Prunus persica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Prunus persica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 isoenzyme A, 0.0016 units, isoenzyme B, 0.011 units, isoenzyme C, 0.0013 units/mg, isoenzyme D, 0.0028 units, 1 unit is defined as absorbance change at 420 nm of 0.001 per min Prunus persica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,5-trihydroxybenzoic acid + O2 isoenzymes A-C Prunus persica ?
-
 ?
3,4,5-trihydroxybenzoic acid + O2 trivial name gallic acid Prunus persica ?
-
 ?
4-methylcatechol + O2 isoenzymes A-D Prunus persica 4-methyl-o-benzoquinone
-
 ?
caffeic acid + 1/2 O2 isoenzymes C and D Prunus persica caffeoyl quinone + H2O
-
 ?
catechol + 1/2 O2 isoenzymes A, B, C and D show no phenolase activity Prunus persica 1,2-benzoquinone + H2O
-
 ?
chlorogenic acid + O2 isoenzymes A-D Prunus persica ?
-
 ?
D-catechin + O2 isoenzymes A-D Prunus persica ?
-
 ?
dopamine + O2 isoenzymes A-D Prunus persica 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
 ?
L-dopa + 1/2 O2 isoenzymes A-D Prunus persica L-dopaquinone + H2O
-
 ?
protocatechuic acid + O2 isoenzyme C Prunus persica ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 half-lives of isoenzymes A, B and D: 5.4, 14,6 and 14.1 min respectively, isoenzyme C: stable for 50 min at 55°C, half-life at 76°C: 2.2 min Prunus persica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 isoenzyme B Prunus persica
6.8
-
 isoenzyme A Prunus persica
7
-
 isoenzyme D Prunus persica
7.2
-
 isoenzyme C Prunus persica