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Literature summary for 1.10.3.1 extracted from
- Molecular cloning and characterisation of grape berry polyphenol oxidase (1994), Plant Mol. Biol., 26, 495-502.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of cDNA | Vitis vinifera |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
1 * 40000, mature enzyme, SDS-PAGE | Vitis vinifera |
| 56700 | - |
1 * 56700, mature enzyme, enzyme is synthesized as a 67300 Da precursor protein, predicted from nucleotide sequence | Vitis vinifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vitis vinifera | P43311 | sultana grape | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hexadecyltrimethylammonium bromide extraction, ammonium sulfate, Q-Sepharose, Phenyl-Sepharose, hydroxylapatite | Vitis vinifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| berry | high expression levels in developing berries and leaves | Vitis vinifera | - |
| leaf | - |
Vitis vinifera | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66 | - |
- |
Vitis vinifera |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, mature enzyme, SDS-PAGE | Vitis vinifera |
| monomer | 1 * 56700, mature enzyme, enzyme is synthesized as a 67300 Da precursor protein, predicted from nucleotide sequence | Vitis vinifera |
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