Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | competitive | Ferula sp. | |
L-ascorbic acid | noncompetitive | Ferula sp. | |
L-cysteine chloride | competitive | Ferula sp. | |
Sodium diethyl dithiocarbamate | competitive | Ferula sp. | |
Sodium metabisulfite | competitive | Ferula sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
(+)-catechin | pH 6.5, 30°C, stem enzyme | Ferula sp. | |
0.554 | - |
(+)-catechin | pH 6.5, 30°C, leaf enzyme | Ferula sp. | |
0.764 | - |
chlorogenic acid | pH 6.0, 20°C, leaf enzyme | Ferula sp. | |
0.798 | - |
(-)-epicatechin | pH 6.0, 15°C, leaf enzyme | Ferula sp. | |
1.07 | - |
chlorogenic acid | pH 6.0, 20°C, stem enzyme | Ferula sp. | |
2.34 | - |
catechol | pH 7.0, 12°C, leaf enzyme | Ferula sp. | |
2.64 | - |
catechol | pH 7.0, 12°C, stem enzyme | Ferula sp. | |
2.89 | - |
(-)-epicatechin | pH 6.0, 15°C, stem enzyme | Ferula sp. | |
6.58 | - |
4-methylcatechol | pH 6.0, 25°C, leaf enzyme | Ferula sp. | |
6.78 | - |
4-methylcatechol | pH 6.0, 25°C, stem enzyme | Ferula sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-catechin + O2 | Ferula sp. | - |
? | - |
? | |
(-)-epicatechin + O2 | Ferula sp. | - |
? | - |
? | |
4-methylcatechol + O2 | Ferula sp. | - |
4-methyl-1,2-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | Ferula sp. | - |
1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | Ferula sp. | - |
? | - |
? | |
additional information | Ferula sp. | polyphenol oxidase is a major enzyme responsible for the browning reaction in damaged plant tissues and fruits | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ferula sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme, 25.8fold from stem and 43.3 from leaves, by (NH4)2SO4 precipitation, dialysis, and gel filtration | Ferula sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ferula sp. | - |
root | - |
Ferula sp. | - |
stem | - |
Ferula sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity, tissue specific activities, overview | Ferula sp. |
13.15 | - |
purified stem enzyme | Ferula sp. |
41.6 | - |
purified leaf enzyme | Ferula sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-catechin + O2 | - |
Ferula sp. | ? | - |
? | |
(-)-epicatechin + O2 | - |
Ferula sp. | ? | - |
? | |
(-)-epicatechin + O2 | high activity | Ferula sp. | ? | - |
? | |
4-methylcatechol + O2 | - |
Ferula sp. | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
catechin + O2 | - |
Ferula sp. | ? | - |
? | |
catechol + 1/2 O2 | - |
Ferula sp. | 1,2-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | best substrate | Ferula sp. | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | - |
Ferula sp. | ? | - |
? | |
dopamine + O2 | low activity | Ferula sp. | ? | - |
? | |
gallic acid + O2 | low activity | Ferula sp. | ? | - |
? | |
L-DOPA + O2 | low activity | Ferula sp. | ? | - |
? | |
additional information | polyphenol oxidase is a major enzyme responsible for the browning reaction in damaged plant tissues and fruits | Ferula sp. | ? | - |
? | |
additional information | substrate specificity, overview, no activity with L-tyrosine | Ferula sp. | ? | - |
? | |
pyrogallol + O2 | low activity | Ferula sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Ferula sp. |
PPO | - |
Ferula sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
12 | 30 | dependent on the substrate, overview | Ferula sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | - |
Ferula sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
60 min, purified enzyme, 40% reduced activity | Ferula sp. |
40 | - |
60 min, purified enzyme, 65% reduced activity | Ferula sp. |
50 | - |
60 min, purified enzyme, complete inactivation | Ferula sp. |
60 | - |
40 min, purified enzyme, irreversible denaturation | Ferula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | dependent on the substrate, overview | Ferula sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Ferula sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Ferula sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0259 | - |
Sodium metabisulfite | pH 7.0, stem enzyme | Ferula sp. | |
0.0454 | - |
Sodium diethyl dithiocarbamate | pH 7.0, stem enzyme | Ferula sp. | |
0.0632 | - |
2-mercaptoethanol | pH 7.0, stem enzyme | Ferula sp. | |
0.0645 | - |
Sodium diethyl dithiocarbamate | pH 7.0, leaf enzyme | Ferula sp. | |
0.0718 | - |
L-ascorbic acid | pH 7.0, stem enzyme | Ferula sp. | |
0.0815 | - |
L-ascorbic acid | pH 7.0, leaf enzyme | Ferula sp. | |
0.116 | - |
L-cysteine chloride | pH 7.0, stem enzyme | Ferula sp. | |
0.119 | - |
Sodium metabisulfite | pH 7.0, leaf enzyme | Ferula sp. | |
0.126 | - |
L-cysteine chloride | pH 7.0, leaf enzyme | Ferula sp. | |
0.138 | - |
2-mercaptoethanol | pH 7.0, leaf enzyme | Ferula sp. |