Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | after the addition of SDS, the catecholoxidase activity of Eurypelma hemocyanin develops gradually over a period of 5 min until a constant activity is reached | Aphonopelma californicum (nom. dub.) |
General Stability | Organism |
---|---|
The activated state of Eurypelma hemocyanin is stable for several hours. | Aphonopelma californicum (nom. dub.) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
dopamine | at high dopamine concentration, a decrease in activity is observed, indicating substrate inhibition | Aphonopelma californicum (nom. dub.) | |
kojic acid | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
L-mimosine | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
Phenylthiourea | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
tyramine | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.22 | - |
dopamine | at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site | Aphonopelma californicum (nom. dub.) | |
1.45 | - |
dopamine | substrate range 0.3-4 mM | Aphonopelma californicum (nom. dub.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Aphonopelma californicum (nom. dub.) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aphonopelma californicum (nom. dub.) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aphonopelma californicum (nom. dub.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 catechol + O2 | - |
Aphonopelma californicum (nom. dub.) | 2 1,2-benzoquinone + 2 H2O | - |
? | |
dopamine + O2 | - |
Aphonopelma californicum (nom. dub.) | dopaminequinone + H2O | - |
? | |
epinephrine + O2 | - |
Aphonopelma californicum (nom. dub.) | ? | - |
? | |
L-dihydroxyphenylalanine + O2 | L-dopa | Aphonopelma californicum (nom. dub.) | L-dopaquinone + H2O | - |
? | |
additional information | to investigate the substrate specificity of activated hemocyanin, the turnover of several diphenols is compared using an oxygen electrode | Aphonopelma californicum (nom. dub.) | ? | - |
? | |
N-acetyldopamine + O2 | NADA | Aphonopelma californicum (nom. dub.) | ? | - |
? | |
norepinephrine + O2 | - |
Aphonopelma californicum (nom. dub.) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
catecholoxidase | - |
Aphonopelma californicum (nom. dub.) |
hemocyanin | - |
Aphonopelma californicum (nom. dub.) |
phenoloxidase | - |
Aphonopelma californicum (nom. dub.) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
dopamine | at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site | Aphonopelma californicum (nom. dub.) | |
6 | - |
dopamine | substrate range 0.3-4 mM | Aphonopelma californicum (nom. dub.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aphonopelma californicum (nom. dub.) |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | fitting attempts reveal that inhibition by phenylthiourea and azide is not in accordance with a competitive binding model, the binding affinity of the inhibitors can not be determined by fitting the data | Aphonopelma californicum (nom. dub.) | |
0.0119 | - |
kojic acid | with dopamine as a substrate | Aphonopelma californicum (nom. dub.) | |
0.0477 | - |
L-mimosine | with dopamine as a substrate | Aphonopelma californicum (nom. dub.) | |
0.2782 | - |
tyramine | with dopamine as a substrate | Aphonopelma californicum (nom. dub.) | |
29.64 | - |
dopamine | at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site | Aphonopelma californicum (nom. dub.) |