Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.1 extracted from

  • Fang, C.; Wang, C.; Xiong, Y.L.; Pomper, K.W.
    Extraction and characterization of polyphenol oxidase in pawpaw (Asimina triloba) fruit (2007), J. Food Biochem., 31, 603-620.
    View publication on PubMed

Application

Application Comment Organism
agriculture could help pawpaw growers and food processors to develop proper storage and processing methods to avoid the undesirable color changes Asimina triloba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
326.6
-
catechol Km of pawpaw fruit PPO is comparable with the Km of PPO from other fruits Asimina triloba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28200
-
PPO exists as two isoforms with molecular weights of 28.2 and 38.3 kDa, SDS-PAGE Asimina triloba
28300
-
PPO exists as two isoforms with molecular weights of 28.2 and 38.3 kDa, SDS-PAGE Asimina triloba

Organism

Organism UniProt Comment Textmining
Asimina triloba
-
pawpaw fruit
-

Purification (Commentary)

Purification (Comment) Organism
purified by gel filtration Asimina triloba

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Asimina triloba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
Asimina triloba o-benzoquinone + H2O
-
?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Asimina triloba
PPO
-
Asimina triloba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5 20 enzyme exhibits the greatest activity in the temperature zone of 5-20°C Asimina triloba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 80 crude enzyme exhibits a maximum activity at 5-20°C, but a brief exposure to 40-80°C results in a rapid decline or complete loss of the enzyme activity Asimina triloba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 PPO activity varies with pH and is the greatest at pH 6.5-7.0 Asimina triloba

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 pH optimum is determined over a pH 4.0-8.5 range Asimina triloba