Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells using pET30c expression vector (high expression) and in Pichia pastoris GS115 using both the secretory and non-secretory vectors pPICZ-A and pPICZA (low expression) | Camellia sinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | A6N8J4 | cultivar Tieguanyin | - |
Camellia sinensis | A6NAA0 | cultivar Longjing | - |
Camellia sinensis | A6YS04 | cultivar Qihong | - |
Camellia sinensis | A6YS05 | cultivar Xiangbolv | - |
Camellia sinensis | C5MLZ1 | cultivar Puer | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, column chromatography, gel filtration | Camellia sinensis |
Renatured (Comment) | Organism |
---|---|
method one is to resuspend the proteins twice in 50 mM Tris-HCl buffer (pH 7.5) with 5 mM dithiothreitol, 2% (v/v) Triton X-100 and 5 mM NaCl, followed by centrifugation at 14000 x g for 5 min. Method two is to resuspend the proteins in one-tenth volume of its original culture of inclusion body isolation buffer containing 20 mM Tris-HCl and 1% (v/v) Triton X-100, pH 7.5, and then in 50 mM Tris-HCl, pH 8.0, each followed by centrifugation at 14000 x g for 5 min | Camellia sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 catechol + O2 | - |
Camellia sinensis | 2 1,2-benzoquinone + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Camellia sinensis |
PPO | - |
Camellia sinensis |
Organism | Comment | Expression |
---|---|---|
Camellia sinensis | a 3.1fold increase in PPO activity over non-recombinant controls is obtained by expressing the PPO fragment without signal sequences and the CuC domain in Escherichia coli BL21 (DE3) using the pET30c vector | up |