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Literature summary for 1.10.3.1 extracted from

  • Palma-Orozco, G.; Ortiz-Moreno, A.; Dorantes-Alvarez, L.; Sampedro, J.G.; Najera, H.
    Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota) (2011), Phytochemistry, 72, 82-88.
    View publication on PubMed
Search for more literature for 1.10.3.1

Activating Compound

Activating Compound Comment Organism Structure
benzoic acid 134% relative activity at 10 mM Pouteria sapota
Citric acid 148% relative activity at 10 mM Pouteria sapota
potassium sorbate 158% relative activity at 10 mM Pouteria sapota

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid one of the most effective inhibitors of isozyme PPO 1, complete inhibition at 0.1 mM Pouteria sapota
EDTA 5% residual activity at 0.1 mM Pouteria sapota
kojic acid 43% residual activity at 1 mM Pouteria sapota
NaCl 29% residual activity at 0.1 mM Pouteria sapota
Sodium metabisulfite one of the most effective inhibitors of isozyme PPO 1, complete inhibition at 0.1 mM Pouteria sapota
succinic acid complete inhibition at 1 mM Pouteria sapota
tropolone 68% residual activity at 0.1 mM Pouteria sapota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 pyrogallol isozyme PPO 1, in 0.2 M sodium phosphate buffer pH 7.0, 25°C Pouteria sapota
44
-
 catechol isozyme PPO 1, in 0.2 M sodium phosphate buffer pH 7.0, 25°C Pouteria sapota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16100
-
 gel filtration Pouteria sapota
18000
-
 1 * 18000, SDS-PAGE Pouteria sapota

Organism

Organism UniProt Comment Textmining
Pouteria sapota
-
 mamey
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, and phenyl Sepharose column chromatography Pouteria sapota

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Pouteria sapota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.32
-
 crude extract, at pH 7.0, 25°C Pouteria sapota
8.76
-
 isozyme PPO 1 after 6.6fold purification, at pH 7.0, 25°C Pouteria sapota
241.5
-
 isozyme PPO 2 after 183fold purification, at pH 7.0, 25°C Pouteria sapota

Storage Stability

Storage Stability Organism
isozyme PPO 2 is highly unstable such as after 24 h of storage PPO 2 activity is not detected Pouteria sapota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2 100% activity with 10 mM substrate concentration Pouteria sapota 2 1,2-benzoquinone + 2 H2O
-
 ?
4-methylcatechol + O2 208% activity with 10 mM substrate concentration compared to catechol Pouteria sapota 4-methyl-1,2-benzoquinone + H2O
-
 ?
additional information the enzyme shows no activity towards caffeic acid, DL-Dopa, ferulic acid and phenol Pouteria sapota ?
-
 ?
pyrogallol + O2 746% activity with 10 mM substrate concentration compared to catechol Pouteria sapota ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 18000, SDS-PAGE Pouteria sapota

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Pouteria sapota
PPO 1 isozyme Pouteria sapota
PPO 2 isozyme Pouteria sapota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 45 optimum temperature for isozyme PPO 1 Pouteria sapota

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 at 20°C the residual activity (26%) is maintained, at higher temperatures the activity decreases (50-60°C) and from 65 to 70°C there is no activity Pouteria sapota

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 PPO is maximum thermally stable up to 35°C Pouteria sapota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 optimum pH for isozyme PPO 1 Pouteria sapota

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 at pH 6.0, isozyme PPO 1 activity increases drastically, reaching maximum activity at pH 7.0 and then again radically decreases at pH 7.5 Pouteria sapota