Cloned (Comment) | Organism |
---|---|
gene ppo-6, expression of N-terminally Strep2-tagged isozyme PPO-6 in Escherichia coli strain Rosetta2(DE3) pLysSRARE2 | Taraxacum officinale |
Protein Variants | Comment | Organism |
---|---|---|
C197S | site-directed mutagenesis of isozyme PPO-6, the C197S-mutein still forms a tetrameric structure but shows impaired enzymatic efficiency and cooperativity and a reduction in stability compared to the wild-type enzyme | Taraxacum officinale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type isozyme PPO-6 displays a low degree in cooperativity in catalysis. Cooperativity of the C197S mutein is significantly decreased at pH 3.5, under SDS activation at pH 5.0 the degree of cooperativity of the C197S mutein is similar to that of the PPO-6 wild-type enzyme | Taraxacum officinale |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a type 3 copper protein | Taraxacum officinale |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, recombinant mutant C197S mutein-tagged isozyme PPO-6, SDS-PAGE | Taraxacum officinale |
60000 | - |
4 * 60000, recombinant wild-type Strep2-tagged isozyme PPO-6, SDS-PAGE | Taraxacum officinale |
120000 | - |
recombinant mutant C197S mutein-tagged isozyme PPO-6, native PAGE | Taraxacum officinale |
250000 | - |
recombinant Strep2-tagged isozyme PPO-6, native PAGE | Taraxacum officinale |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 o-diphenol + O2 | Taraxacum officinale | - |
2 o-quinone + 2 H2O | - |
? | |
additional information | Taraxacum officinale | the enzyme catalyzes the oxidation of ortho-diphenols to the corresponding quinones | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taraxacum officinale | I7HUF2 | PPO-6; gene ppo-6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-methylcatechol + O2 | - |
Taraxacum officinale | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 o-diphenol + O2 | - |
Taraxacum officinale | 2 o-quinone + 2 H2O | - |
? | |
2 o-diphenol + O2 | the reaction depends on molecular oxygen, which is reduced by the copper-containing catalytic domain of the enzyme | Taraxacum officinale | 2 o-quinone + 2 H2O | - |
? | |
additional information | the enzyme catalyzes the oxidation of ortho-diphenols to the corresponding quinones | Taraxacum officinale | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, recombinant mutant C197S mutein-tagged isozyme PPO-6, SDS-PAGE | Taraxacum officinale |
More | isozyme PPO-6 is a tetramer, the surface-exposed Cys197 residue stabilizes the tetramer via a disulfide linkage | Taraxacum officinale |
tetramer | 4 * 60000, recombinant wild-type Strep2-tagged isozyme PPO-6, SDS-PAGE | Taraxacum officinale |
Synonyms | Comment | Organism |
---|---|---|
PPO-6 | - |
Taraxacum officinale |
General Information | Comment | Organism |
---|---|---|
additional information | modeling of the three-dimensional structure of the PPO-6 tyrosinase domain | Taraxacum officinale |