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Literature summary for 1.10.3.1 extracted from

  • Liu, F.; Zhao, J.H.; Wen, X.; Ni, Y.Y.
    Purification and structural analysis of membrane-bound polyphenol oxidase from Fuji apple (2015), Food Chem., 183, 72-77 .
    View publication on PubMed
Search for more literature for 1.10.3.1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Malus domestica 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper enzyme Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-methylcatechol + O2 Malus domestica
-
 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 Malus domestica
-
 2 1,2-benzoquinone + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Malus domestica
-
 cv. Red Fuji
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Purification (Commentary)

Purification (Comment) Organism
native enzyme 54.41fold by temperature-induced phase partitioning technique, dialysis, and ion exchange chromatography Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Malus domestica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 370004 U/mg for the purified native enzyme. One unit of enzyme activity is defined as a 0.001-unit change in absorbance per min per ml Malus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-methylcatechol + O2
-
 Malus domestica 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
2 4-methylcatechol + O2 best substrate Malus domestica 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Malus domestica 2 1,2-benzoquinone + 2 H2O
-
 ?
additional information the putative substrate-binding pocket contains six polar or charged amino acids, His191, His221, Trp224, Trp228, Phe227, and Val190. Trp224 and Trp228 form hydrogen bonds with 4-methylcatechol. Molecular docking of optimum substrate of mPPO, 4-methylcatechol, overview Malus domestica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67300, SDS-PAGE Malus domestica
More the three-dimensional structure of mPPO consists of six alpha-helices, two short beta-strands, and ten random coils, three-dimensional structure modeling based on the Vitis vinifera PPO, PDB ID 2P3X, as template Malus domestica

Synonyms

Synonyms Comment Organism
MppO
-
 Malus domestica
polyphenol oxidase
-
 Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Malus domestica

General Information

General Information Comment Organism
additional information three-dimensional structure modeling based on the Vitis vinifera PPO, PDB ID 2P3X, as template Malus domestica