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Literature summary for 1.10.3.11 extracted from

  • Hoefnagel, M.; Rich, P.R.; Zhang, Q.; Wiskich, J.T.
    Substrate kinetics of the plant mitochondrial alternative oxidase and the effects of pyruvate (1997), Plant Physiol., 115, 1145-1153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDT-20 the addition of 0.025% (v/v) EDT-20 in the assay increases the activity 3-4fold Glycine max
EDT-20 the addition of 0.025% (v/v) EDT-20 in the assay increases the activity 3-4fold Arum italicum
pyruvate potent activator, its presence is essential for maximum activity, pyruvate increases the activity of AOX but does not increase its affinity for its substrate Glycine max
pyruvate potent activator, its presence is essential for maximum activity, pyruvate increases the activity of AOX but does not increase its affinity for its substrate Arum italicum

Inhibitors

Inhibitors Comment Organism Structure
n-octyl gallate the enzyme is fully sensitive to 0.001 mM octyl gallate Arum italicum
n-octyl gallate the enzyme is fully sensitive to 0.001 mM octyl gallate Glycine max
n-propyl gallate
-
Arum italicum
n-propyl gallate
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1153
-
ubiquinol-1 in the presence of EDT-20 (0.025%) and pyruvate, 0.3 M succinate, 25 mM TES, 10 mM potassium phosphate, and 2 mM magnesium sulfate, pH 7.0, at 25°C Glycine max
0.331
-
ubiquinol-1 in the presence of EDT-20 (0.025%) and pyruvate, 0.3 M succinate, 25 mM TES, 10 mM potassium phosphate, and 2 mM magnesium sulfate, pH 7.0, at 25°C Arum italicum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane inner mitochondrial membrane Glycine max 31966
-
mitochondrial membrane inner mitochondrial membrane Arum italicum 31966
-

Organism

Organism UniProt Comment Textmining
Arum italicum
-
-
-
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Q-Sepharose column chromatography Arum italicum

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Glycine max
-
spadix
-
Arum italicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decylubiquinol + O2
-
Glycine max decylubiquinone + H2O
-
?
decylubiquinol + O2
-
Arum italicum decylubiquinone + H2O
-
?
duroquinol + O2
-
Glycine max duroquinone + H2O
-
?
duroquinol + O2
-
Arum italicum duroquinone + H2O
-
?
ubiquinol-1 + O2 most suitable substrate Glycine max ubiquinone-1 + H2O
-
?
ubiquinol-1 + O2 most suitable substrate Arum italicum ubiquinone-1 + H2O
-
?

Synonyms

Synonyms Comment Organism
alternative oxidase
-
Glycine max
alternative oxidase
-
Arum italicum
AOX
-
Glycine max
AOX
-
Arum italicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Glycine max
7 7.5
-
Arum italicum