Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Schizosaccharomyces pombe strain Sp.011, subcloning in Escherichia coli strains JM101 and 110 | Sauromatum venosum |
Protein Variants | Comment | Organism |
---|---|---|
C172A | site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme | Sauromatum venosum |
T179A | site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme | Sauromatum venosum |
W206F | site-directed mutagenesis, inactive mutant | Sauromatum venosum |
W206Y | site-directed mutagenesis, inactive mutant | Sauromatum venosum |
Y253F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Sauromatum venosum |
Y299F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Sauromatum venosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
octyl gallate | - |
Sauromatum venosum | |
sialic acid | - |
Sauromatum venosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
O2 | mutant T179A, pH and temperature not specified in the publication | Sauromatum venosum | |
0.0092 | - |
O2 | mutant C172A, pH and temperature not specified in the publication | Sauromatum venosum | |
0.017 | - |
O2 | mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | |
0.018 | - |
O2 | wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | |
0.0205 | - |
O2 | mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sauromatum venosum | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ubiquinol + O2 | Sauromatum venosum | - |
2 ubiquinone + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sauromatum venosum | P22185 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ubiquinol + O2 | - |
Sauromatum venosum | 2 ubiquinone + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alternative oxidase | - |
Sauromatum venosum |
AOX | - |
Sauromatum venosum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00007 | - |
mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
0.00008 | - |
mutants C172A and T179A, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
0.00009 | - |
wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
0.0001 | - |
mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
0.012 | - |
mutant T179A, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
0.016 | - |
wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
0.018 | - |
mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
0.019 | - |
mutant C172A, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
0.02 | - |
mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is found in mitochondria of all higher plants studied to date | Sauromatum venosum |
physiological function | the alternative oxidase (AOX) is a non-protonmotive ubiquinol oxidase | Sauromatum venosum |