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Literature summary for 1.10.3.11 extracted from

  • Young, L.; Rosell-Hidalgo, A.; Inaoka, D.K.; Xu, F.; Albury, M.; May, B.; Kita, K.; Moore, A.L.
    Kinetic and structural characterisation of the ubiquinol-binding site and oxygen reduction by the trypanosomal alternative oxidase (2020), Biochim. Biophys. Acta Bioenerg., 1861, 148247 .
    View publication on PubMed
Search for more literature for 1.10.3.11

Protein Variants

Protein Variants Comment Organism
D100A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 8.8fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
D100E kcat/Km-value of the mutant enzyme for ubiquinol-1 is 5.7fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
E215A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 34fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
E215D kcat/Km-value of the mutant enzyme for ubiquinol-1 is 528fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
E215N kcat/Km-value of the mutant enzyme for ubiquinol-1 is 567fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
L122A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 34.3fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
L212A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 31.2fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
R118A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 22.5fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
R118K kcat/Km-value of the mutant enzyme for ubiquinol-1 is 19.6fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
R96A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 7.9fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
R96K kcat/Km-value of the mutant enzyme for ubiquinol-1 is 7.8fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
T219A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 36.4fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
T219S kcat/Km-value of the mutant enzyme for ubiquinol-1 is 89.1fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
ascochlorin
-
 Trypanosoma brucei brucei
ascofuranone
-
 Trypanosoma brucei brucei
colletochlorin B
-
 Trypanosoma brucei brucei
colletochlorin D
-
 Trypanosoma brucei brucei
octyl-gallate
-
 Trypanosoma brucei brucei
salicylic hydroxamic acid
-
 Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0488
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei
0.0613
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei
0.068
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118K Trypanosoma brucei brucei
0.0782
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei
0.0783
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118A Trypanosoma brucei brucei
0.113
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219A Trypanosoma brucei brucei
0.1217
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei
0.1463
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L122A Trypanosoma brucei brucei
0.1697
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219S Trypanosoma brucei brucei
0.1703
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei
0.3374
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215A Trypanosoma brucei brucei
0.339
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei
0.3473
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215N Trypanosoma brucei brucei
0.4295
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215D Trypanosoma brucei brucei
5.5
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme E215D Trypanosoma brucei brucei
6.2
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei
8.1
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei
8.5
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R118A Trypanosoma brucei brucei
12
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme E215N Trypanosoma brucei brucei
12.9
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme E215A Trypanosoma brucei brucei
13.4
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme T219A Trypanosoma brucei brucei
13.6
-
 O2 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei
14.7
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei
14.8
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei
15
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R118K Trypanosoma brucei brucei
15.3
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei
16.8
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme L122A Trypanosoma brucei brucei
17.3
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme T219S Trypanosoma brucei brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ubiquinol-1 + O2 Trypanosoma brucei brucei
-
 2 ubiquinone-1 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q26710
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ubiquinol-1 + O2
-
 Trypanosoma brucei brucei 2 ubiquinone-1 + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
trypanosomal alternative oxidase
-
 Trypanosoma brucei brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000154
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei colletochlorin D
0.0000023
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei octyl-gallate
0.0000037
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei salicylic hydroxamic acid
0.0000044
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei salicylic hydroxamic acid
0.0000044
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei salicylic hydroxamic acid
0.0000052
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei octyl-gallate
0.0000061
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei salicylic hydroxamic acid
0.000007
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei ascofuranone
0.0000074
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei ascochlorin
0.0000075
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei colletochlorin B
0.000022
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei salicylic hydroxamic acid
0.000033
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei colletochlorin D
0.000036
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei colletochlorin B
0.000038
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei ascofuranone
0.000046
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei octyl-gallate
0.000051
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei ascofuranone
0.000051
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei colletochlorin D
0.000058
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei ascofuranone
0.000061
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei colletochlorin D
0.000066
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei colletochlorin B
0.000081
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei colletochlorin D
0.000089
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei ascofuranone
0.00009
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei colletochlorin D
0.000129
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei ascofuranone
0.000137
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei colletochlorin B
0.000167
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei ascochlorin
0.000203
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei ascochlorin
0.000215
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei ascochlorin
0.000221
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei ascochlorin
0.00023
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei octyl-gallate
0.000239
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei colletochlorin B
0.000308
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei octyl-gallate
0.000316
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei ascochlorin
0.00047
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei colletochlorin B
0.000862
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei octyl-gallate
0.047
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei salicylic hydroxamic acid

General Information

General Information Comment Organism
physiological function the enzyme plays a central role in metabolism through facilitating the turnover of the TCA cycle whilst reducing ROS production Trypanosoma brucei brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.62
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215N Trypanosoma brucei brucei
1.74
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215D Trypanosoma brucei brucei
10.3
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219S Trypanosoma brucei brucei
25.2
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219A Trypanosoma brucei brucei
26.7
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L122A Trypanosoma brucei brucei
27
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215A Trypanosoma brucei brucei
29.4
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei
40.8
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118A Trypanosoma brucei brucei
46.6
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118K Trypanosoma brucei brucei
104
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei
116
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei
117
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei
162
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei
918
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei