BRENDA - Enzyme Database show
show all sequences of 1.10.3.15

A novel o-aminophenol oxidase responsible for formation of the phenoxazinone chromophore of grixazone

Suzuki, H.; Furusho, Y.; Higashi, T.; Ohnishi, Y.; Horinouchi, S.; J. Biol. Chem. 281, 824-833 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
GriE protein
GriE activates the enzyme by transferring copper ions
Streptomyces griseus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells
Streptomyces griseus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Nitrophenol
-
Streptomyces griseus
3-amino-4-hydroxybenzensulfonic acid
-
Streptomyces griseus
4-hydroxy-3-nitrobenzaldehyde
-
Streptomyces griseus
4-hydroxybenzaldehyde
-
Streptomyces griseus
aniline
-
Streptomyces griseus
L-tyrosine
-
Streptomyces griseus
additional information
10 mM EDTA and o-phenanthroline have negligible effects on the purified enzyme
Streptomyces griseus
phenol
-
Streptomyces griseus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
3,4-Dihydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
0.58
-
3-amino-4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
0.75
-
2-amino-4-methylphenol
at pH 7.0 and 55C
Streptomyces griseus
3.5
-
o-aminophenol
at pH 7.0 and 55C
Streptomyces griseus
5.5
-
L-Dopa
at pH 7.0 and 55C
Streptomyces griseus
19
-
catechol
at pH 7.0 and 55C
Streptomyces griseus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
the enzyme contains two copper ions in the active site. 10 mM Cu2+ specifically increases the enzyme activity by about 20fold
Streptomyces griseus
additional information
Zn2+, Ni2+, Co2+, Mn2+, Fe2+, or Mg2+ exert almost no effects
Streptomyces griseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE
Streptomyces griseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
Streptomyces griseus
-
grixazone B + H2O + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces griseus
-
-
-
Streptomyces griseus IFO 13350
-
-
-
Purification (Commentary)
Commentary
Organism
Cu2+-charged HiTrap chelating column chromatography, HiTrap Q column chromatography, Resource PHE column chromatography, and Superdex 200 gel filtration
Streptomyces griseus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0000035
-
crude extract, at pH 7.0 and 30C
Streptomyces griseus
0.0658
-
after 19000fold purification, at pH 7.0 and 30C
Streptomyces griseus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-4-methylphenol + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
3,4-dihydroxybenzaldehyde + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
3-amino-4-hydroxybenzaldehyde + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
grixazone A + H2O + CO2
-
-
-
?
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
grixazone B + H2O + CO2
-
-
-
?
4-amino-3-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
catechol + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
L-Dopa + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
additional information
the enzyme shows no monophenolase activity, although it oxidizes various o-aminophenols as preferable substrates rather than catechol-type substrates. The enzyme does not use tyrosine as a substrate
674768
Streptomyces griseus
?
-
-
-
-
o-aminophenol + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
2-aminophenoxazin-3-one + H2O + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Streptomyces griseus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
at pH 7.0
Streptomyces griseus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
40
70
more than 50% activity between 30 and 70C
Streptomyces griseus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
10
55
when the enzyme sample is kept at pH 7.0 for 20 min, it remains active below 55C, and activity drops sharply above 55C
Streptomyces griseus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.066
-
L-Dopa
at pH 7.0 and 55C
Streptomyces griseus
0.8
-
3,4-Dihydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
12
-
catechol
at pH 7.0 and 55C
Streptomyces griseus
14
-
3-amino-4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
18
-
2-amino-4-methylphenol
at pH 7.0 and 55C
Streptomyces griseus
20
-
o-aminophenol
at pH 7.0 and 55C
Streptomyces griseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10.5
-
Streptomyces griseus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
more than 50% activity between pH 6.0 and 11.0
Streptomyces griseus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
8.5
the enzyme is most stable at pH 6.5-8.5
Streptomyces griseus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
3.2
-
L-tyrosine
at pH 7.0 and 55C
Streptomyces griseus
3.9
-
4-hydroxy-3-nitrobenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
6.1
-
2-Nitrophenol
at pH 7.0 and 55C
Streptomyces griseus
14
-
aniline
at pH 7.0 and 55C
Streptomyces griseus
21
-
phenol
at pH 7.0 and 55C
Streptomyces griseus
31
-
3-amino-4-hydroxybenzensulfonic acid
at pH 7.0 and 55C
Streptomyces griseus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
GriE protein
GriE activates the enzyme by transferring copper ions
Streptomyces griseus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells
Streptomyces griseus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Nitrophenol
-
Streptomyces griseus
3-amino-4-hydroxybenzensulfonic acid
-
Streptomyces griseus
4-hydroxy-3-nitrobenzaldehyde
-
Streptomyces griseus
4-hydroxybenzaldehyde
-
Streptomyces griseus
aniline
-
Streptomyces griseus
L-tyrosine
-
Streptomyces griseus
additional information
10 mM EDTA and o-phenanthroline have negligible effects on the purified enzyme
Streptomyces griseus
phenol
-
Streptomyces griseus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
3.2
-
L-tyrosine
at pH 7.0 and 55C
Streptomyces griseus
3.9
-
4-hydroxy-3-nitrobenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
6.1
-
2-Nitrophenol
at pH 7.0 and 55C
Streptomyces griseus
14
-
aniline
at pH 7.0 and 55C
Streptomyces griseus
21
-
phenol
at pH 7.0 and 55C
Streptomyces griseus
31
-
3-amino-4-hydroxybenzensulfonic acid
at pH 7.0 and 55C
Streptomyces griseus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
3,4-Dihydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
0.58
-
3-amino-4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
0.75
-
2-amino-4-methylphenol
at pH 7.0 and 55C
Streptomyces griseus
3.5
-
o-aminophenol
at pH 7.0 and 55C
Streptomyces griseus
5.5
-
L-Dopa
at pH 7.0 and 55C
Streptomyces griseus
19
-
catechol
at pH 7.0 and 55C
Streptomyces griseus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
the enzyme contains two copper ions in the active site. 10 mM Cu2+ specifically increases the enzyme activity by about 20fold
Streptomyces griseus
additional information
Zn2+, Ni2+, Co2+, Mn2+, Fe2+, or Mg2+ exert almost no effects
Streptomyces griseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE
Streptomyces griseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
Streptomyces griseus
-
grixazone B + H2O + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Cu2+-charged HiTrap chelating column chromatography, HiTrap Q column chromatography, Resource PHE column chromatography, and Superdex 200 gel filtration
Streptomyces griseus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0000035
-
crude extract, at pH 7.0 and 30C
Streptomyces griseus
0.0658
-
after 19000fold purification, at pH 7.0 and 30C
Streptomyces griseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-4-methylphenol + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
3,4-dihydroxybenzaldehyde + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
3-amino-4-hydroxybenzaldehyde + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
grixazone A + H2O + CO2
-
-
-
?
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
grixazone B + H2O + CO2
-
-
-
?
4-amino-3-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
catechol + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
L-Dopa + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
?
-
-
-
?
additional information
the enzyme shows no monophenolase activity, although it oxidizes various o-aminophenols as preferable substrates rather than catechol-type substrates. The enzyme does not use tyrosine as a substrate
674768
Streptomyces griseus
?
-
-
-
-
o-aminophenol + N-acetyl-L-cysteine + O2
-
674768
Streptomyces griseus
2-aminophenoxazin-3-one + H2O + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Streptomyces griseus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
at pH 7.0
Streptomyces griseus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
40
70
more than 50% activity between 30 and 70C
Streptomyces griseus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
10
55
when the enzyme sample is kept at pH 7.0 for 20 min, it remains active below 55C, and activity drops sharply above 55C
Streptomyces griseus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.066
-
L-Dopa
at pH 7.0 and 55C
Streptomyces griseus
0.8
-
3,4-Dihydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
12
-
catechol
at pH 7.0 and 55C
Streptomyces griseus
14
-
3-amino-4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
18
-
2-amino-4-methylphenol
at pH 7.0 and 55C
Streptomyces griseus
20
-
o-aminophenol
at pH 7.0 and 55C
Streptomyces griseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10.5
-
Streptomyces griseus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
more than 50% activity between pH 6.0 and 11.0
Streptomyces griseus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
8.5
the enzyme is most stable at pH 6.5-8.5
Streptomyces griseus
General Information
General Information
Commentary
Organism
metabolism
GriF is an o-aminophenol oxidase responsible for the in vivo formation of the phenoxazinone chromophore in the grixazone biosynthetic pathway
Streptomyces griseus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
GriF is an o-aminophenol oxidase responsible for the in vivo formation of the phenoxazinone chromophore in the grixazone biosynthetic pathway
Streptomyces griseus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0073
-
4-Amino-3-hydroxybenzoate
at pH 7.0 and 55C
Streptomyces griseus
0.012
-
L-Dopa
at pH 7.0 and 55C
Streptomyces griseus
0.19
-
3-amino-4-hydroxybenzoate
at pH 7.0 and 55C
Streptomyces griseus
0.32
-
catechol
at pH 7.0 and 55C
Streptomyces griseus
0.97
-
3,4-Dihydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
5.8
-
o-aminophenol
at pH 7.0 and 55C
Streptomyces griseus
23
-
2-amino-4-methylphenol
at pH 7.0 and 55C
Streptomyces griseus
24
-
3-amino-4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0073
-
4-Amino-3-hydroxybenzoate
at pH 7.0 and 55C
Streptomyces griseus
0.012
-
L-Dopa
at pH 7.0 and 55C
Streptomyces griseus
0.19
-
3-amino-4-hydroxybenzoate
at pH 7.0 and 55C
Streptomyces griseus
0.32
-
catechol
at pH 7.0 and 55C
Streptomyces griseus
0.97
-
3,4-Dihydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
5.8
-
o-aminophenol
at pH 7.0 and 55C
Streptomyces griseus
23
-
2-amino-4-methylphenol
at pH 7.0 and 55C
Streptomyces griseus
24
-
3-amino-4-hydroxybenzaldehyde
at pH 7.0 and 55C
Streptomyces griseus
Other publictions for EC 1.10.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713565
Le Roes-Hill
Phenoxazinone synthase: whats' ...
Streptomyces griseus subsp. griseus
Trends Biotechnol.
27
248-258
2009
-
-
-
-
-
-
7
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
674768
Suzuki
A novel o-aminophenol oxidase ...
Streptomyces griseus, Streptomyces griseus IFO 13350
J. Biol. Chem.
281
824-833
2006
1
-
1
-
-
-
8
6
-
2
1
1
-
6
-
-
1
-
-
-
2
-
9
1
1
1
1
6
1
1
1
-
7
-
-
1
-
1
-
-
-
-
-
8
7
6
-
2
1
1
-
-
-
1
-
-
2
-
9
1
1
1
1
6
1
1
1
-
-
1
1
-
8
8
722650
Suzuki
Novel benzene ring biosynthesi ...
Streptomyces griseus, Streptomyces griseus IFO 13350
J. Biol. Chem.
281
36944-36951
2006
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-