Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.16 extracted from

  • Xu, N.; Ahuja, E.G.; Janning, P.; Mavrodi, D.V.; Thomashow, L.S.; Blankenfeldt, W.
    Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis (2013), Acta Crystallogr. Sect. D, 69, 1403-1413.
    View publication on PubMed
Search for more literature for 1.10.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens
expression in Escherichia coli Burkholderia lata

Crystallization (Commentary)

Crystallization (Comment) Organism
complex with excess FMN and with the phenazine-biosynthesis intermediates hexahydrophenazine-1,6-dicarboxylate and tetrahydrophenazine-1-carboxylate generated in situ Pseudomonas fluorescens
complex with excess FMN and with the phenazine-biosynthesis intermediates hexahydrophenazine-1,6-dicarboxylate and tetrahydrophenazine-1-carboxylate generated in situ Burkholderia lata

Organism

Organism UniProt Comment Textmining
Burkholderia lata Q396C5
-
-
Burkholderia lata ATCC 17760 Q396C5
-
-
Pseudomonas fluorescens Q51793
-
-
Pseudomonas fluorescens 2-79 Q51793
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + O2
-
 Pseudomonas fluorescens (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + O2
-
 Burkholderia lata (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + O2
-
 Burkholderia lata ATCC 17760 (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + O2
-
 Pseudomonas fluorescens 2-79 (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylic acid + O2
-
 Pseudomonas fluorescens (10aS)-10,10a-dihydrophenazine-1-carboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylic acid + O2
-
 Burkholderia lata (10aS)-10,10a-dihydrophenazine-1-carboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylic acid + O2
-
 Burkholderia lata ATCC 17760 (10aS)-10,10a-dihydrophenazine-1-carboxylic acid + H2O2
-
 ?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylic acid + O2
-
 Pseudomonas fluorescens 2-79 (10aS)-10,10a-dihydrophenazine-1-carboxylic acid + H2O2
-
 ?
(1R,5aS,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylic acid + O2
-
 Pseudomonas fluorescens (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,5aS,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylic acid + O2
-
 Burkholderia lata (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,5aS,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylic acid + O2
-
 Burkholderia lata ATCC 17760 (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
(1R,5aS,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylic acid + O2
-
 Pseudomonas fluorescens 2-79 (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + H2O2
-
 ?
additional information PhzG is the terminal enzyme in phenazine biosynthesis and its relaxed substrate specificity lets it participate in the generation of both phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid. Competition between flavin-dependent oxidations through PhzG and spontaneous oxidative decarboxylations determines the ratio of phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid and unsubstituted phenazine as the products of phenazine biosynthesis. PhzG synthesizes phenazines in their reduced form Pseudomonas fluorescens ?
-
 ?
additional information PhzG is the terminal enzyme in phenazine biosynthesis and its relaxed substrate specificity lets it participate in the generation of both phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid. Competition between flavin-dependent oxidations through PhzG and spontaneous oxidative decarboxylations determines the ratio of phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid and unsubstituted phenazine as the products of phenazine biosynthesis. PhzG synthesizes phenazines in their reduced form Burkholderia lata ?
-
 ?
additional information PhzG is the terminal enzyme in phenazine biosynthesis and its relaxed substrate specificity lets it participate in the generation of both phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid. Competition between flavin-dependent oxidations through PhzG and spontaneous oxidative decarboxylations determines the ratio of phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid and unsubstituted phenazine as the products of phenazine biosynthesis. PhzG synthesizes phenazines in their reduced form Burkholderia lata ATCC 17760 ?
-
 ?
additional information PhzG is the terminal enzyme in phenazine biosynthesis and its relaxed substrate specificity lets it participate in the generation of both phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid. Competition between flavin-dependent oxidations through PhzG and spontaneous oxidative decarboxylations determines the ratio of phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid and unsubstituted phenazine as the products of phenazine biosynthesis. PhzG synthesizes phenazines in their reduced form Pseudomonas fluorescens 2-79 ?
-
 ?

Synonyms

Synonyms Comment Organism
Bcep18194_B1572
-
 Burkholderia lata
phzG
-
 Pseudomonas fluorescens
phzG
-
 Burkholderia lata

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Pseudomonas fluorescens
FMN
-
 Burkholderia lata