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Literature summary for 1.10.3.2 extracted from

  • Ranocha, P.; McDougall, G.; Hawkins, S.; Sterjiades, R.; Borderies, G.; Stewart, D.; Cabanes-MacHeteau, M.; Boudet, A.M.; Goffner, D.
    Biochemical characterization, molecular cloning and expression of laccases - a divergent gene family - in poplar (1999), Eur. J. Biochem., 259, 485-495.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
lac90, lac110 and three additional cDNAs Populus x canadensis

Inhibitors

Inhibitors Comment Organism Structure
azide 55-70% inhibition at 0.5 mM Populus x canadensis
CN- 40-65% inhibition at 0.5 mM Populus x canadensis
sodium dodecylsulfate 20-70% inhibition at 8 mM Populus x canadensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 hydroquinone isoenzyme lac90 Populus x canadensis
0.002
-
 coniferyl alcohol isoenzyme lac90 Populus x canadensis
0.02
-
 p-coumaryl alcohol isoenzyme lac90 Populus x canadensis
0.03
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) isoenzyme lac90 Populus x canadensis
0.03
-
 coniferaldehyde isoenzyme lac90 Populus x canadensis
1.56
-
 4-methyl catechol isoenzyme lac90 Populus x canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 x * 90000, SDS-PAGE, lac90, x * 110000, SDS-PAGE, lac110 Populus x canadensis
110000
-
 x * 90000, SDS-PAGE, lac90, x * 110000, SDS-PAGE, lac110 Populus x canadensis

Organism

Organism UniProt Comment Textmining
Populus x canadensis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Populus x canadensis

Purification (Commentary)

Purification (Comment) Organism
-
 Populus x canadensis

Source Tissue

Source Tissue Comment Organism Textmining
xylem
-
 Populus x canadensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Populus x canadensis ?
-
 ?
4-methylcatechol + O2
-
 Populus x canadensis 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
 ?
coniferaldehyde + O2
-
 Populus x canadensis ?
-
 ?
coniferyl alcohol + O2
-
 Populus x canadensis ? + H2O
-
 ?
p-coumaryl alcohol + O2
-
 Populus x canadensis ?
-
 ?
quinol + O2
-
 Populus x canadensis ? + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE, lac90, x * 110000, SDS-PAGE, lac110 Populus x canadensis