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Literature summary for 1.10.3.2 extracted from

  • Endo, K.; Hayashi, Y.; Hibi, T.; Hosono, K.; Beppu, T.; Ueda, K.
    Enzymological characterization of EpoA, a laccase-like phenol oxidase produced by Streptomyces griseus (2003), J. Biochem., 133, 671-677.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
2,2-bipyridyl 1 mM, 61% residual activity Streptomyces griseus
2,4-dithio-6-pyrimidine 1 mM, 31% residual activity Streptomyces griseus
bathocuproinedisulfonic acid 1 mM, 39% residual activity Streptomyces griseus
dimethyldithiocarbamate trihydrate 1 mM, 13% residual activity Streptomyces griseus
EDTA 1 mM, 67% residual activity Streptomyces griseus
hydroxylamine 1 mM, 62% residual activity Streptomyces griseus
Sodium azide 1 mM, 68% residual activity Streptomyces griseus
Sodium cyanide 1 mM, 0% residual activity Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 N,N-dimethyl-p-phenylenediamine sulfate pH 6.5, 40°C Streptomyces griseus

Metals/Ions

Metals/Ions Comment Organism Structure
copper four ions per subunit Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 3 * 38000, SDS-PAGE after boiling for 20 min, 114000, stable migration in regular SDS-PAGE Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
 expression in Escherichia coli, C-terminal His-tag
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoantipyrine + O2 19% of the activity with N,N-dimethyl-p-phenylenediamine sulfate Streptomyces griseus ? + H2O
-
 ?
chlorogenic acid + O2 26.5% of the activity with N,N-dimethyl-p-phenylenediamine sulfate Streptomyces griseus ? + H2O
-
 ?
dihydroxyphenylalanine + O2 23.6% of the activity with N,N-dimethyl-p-phenylenediamine sulfate Streptomyces griseus ? + H2O
-
 ?
m-methoxyphenol + O2 15% of the activity with N,N-dimethyl-p-phenylenediamine sulfate Streptomyces griseus ? + H2O
-
 ?
N,N-dimethyl-p-phenylenediamine sulfate + O2
-
 Streptomyces griseus ? + H2O
-
 ?
p-phenylenediamine + O2 33% of the activity with N,N-dimethyl-p-phenylenediamine sulfate Streptomyces griseus ? + H2O
-
 ?
pyrogallol + O2 22% of the activity with N,N-dimethyl-p-phenylenediamine sulfate Streptomyces griseus ? + H2O
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 38000, SDS-PAGE after boiling for 20 min, 114000, stable migration in regular SDS-PAGE Streptomyces griseus

Synonyms

Synonyms Comment Organism
EpoA
-
 Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 60 min, 40% residual activity Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptomyces griseus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces griseus three isomers
-
 4.9