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Literature summary for 1.10.3.2 extracted from

  • Rebrikov, D.N.; Stepanova, E.V.; Koroleva, O.V.; Budarina, Z.I.; Zakharova, M.V.; Yurkova, T.V.; Solonin, A.S.; Belova, O.V.; Pozhidaeva, Z.A.; Leontevsky, A.A.
    Laccase of the lignolytic fungus Trametes hirsuta: purification and characterization of the enzyme, and cloning and primary structure of the gene (2006), Appl. Biochem. Microbiol., 42, 564-572.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, primary structure of the gene, sequence comparisons Trametes hirsuta

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trametes hirsuta
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ type 1 copper ion, Redox potential: 790 mV, 4 ions per enzyme molecule Trametes hirsuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE Trametes hirsuta

Organism

Organism UniProt Comment Textmining
Trametes hirsuta
-
strain 072, a lignolytic basidiomycete
-
Trametes hirsuta 72
-
strain 072, a lignolytic basidiomycete
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12% carbohydrate content, sugar chain are composed of mannose and N-acetyl-glycosamine Trametes hirsuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity, overview Trametes hirsuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eugenol + O2
-
Trametes hirsuta ?
-
?
eugenol + O2
-
Trametes hirsuta 72 ?
-
?
guaiacol + O2
-
Trametes hirsuta ?
-
?
guaiacol + O2
-
Trametes hirsuta 72 ?
-
?
homovanillic acid + O2
-
Trametes hirsuta ?
-
?
homovanillic acid + O2
-
Trametes hirsuta 72 ?
-
?
hydroquinone + O2
-
Trametes hirsuta ?
-
?
hydroquinone + O2
-
Trametes hirsuta 72 ?
-
?
K4[Fe(CN)]6 + O2
-
Trametes hirsuta ?
-
?
phenol + O2
-
Trametes hirsuta ?
-
?
pyrocatechol + O2
-
Trametes hirsuta ?
-
?
pyrocatechol + O2
-
Trametes hirsuta 72 ?
-
?
sinapic acid + O2
-
Trametes hirsuta ?
-
?
vanillic acid + O2
-
Trametes hirsuta ?
-
?
vanillic alcohol + O2
-
Trametes hirsuta ?
-
?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Trametes hirsuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Trametes hirsuta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38
-
phenol pH 4.5, 55°C Trametes hirsuta
102
-
vanillic acid pH 4.5, 55°C Trametes hirsuta
352
-
vanillic alcohol pH 4.5, 55°C Trametes hirsuta
403
-
o-benzenediol pH 4.5, 55°C Trametes hirsuta
404
-
K4[Fe(CN)]6 pH 4.5, 55°C Trametes hirsuta
408
-
homovanillic acid pH 4.5, 55°C Trametes hirsuta
424
-
guaiacol pH 4.5, 55°C Trametes hirsuta
428
-
eugenol pH 4.5, 55°C Trametes hirsuta
455
-
hydroquinone pH 4.5, 55°C Trametes hirsuta
578
-
Sinapic acid pH 4.5, 55°C Trametes hirsuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Trametes hirsuta

pI Value

Organism Comment pI Value Maximum pI Value
Trametes hirsuta
-
-
4