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Literature summary for 1.10.3.2 extracted from

  • Wang, H.X.; Ng, T.B.
    Purification of a laccase from fruiting bodies of the mushroom Pleurotus eryngii (2006), Appl. Microbiol. Biotechnol., 69, 521-525.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 34000, SDS-PAGE Pleurotus eryngii
68000
-
gel filtration Pleurotus eryngii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pleurotus eryngii the laccase exhibits an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 0.0022 mM ?
-
?

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme about 35fold from fruiting bodies by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration Pleurotus eryngii

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Pleurotus eryngii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the laccase exhibits an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 0.0022 mM Pleurotus eryngii ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Pleurotus eryngii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Pleurotus eryngii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 high activity at 80°C, optimum at 70°C, constant rise of activity from 20°C to 70°C Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Pleurotus eryngii

pH Range

pH Minimum pH Maximum Comment Organism
3 6 abrupt activity decline at pH 6.0, indiscernible at pH 8.0-9.0 Pleurotus eryngii