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Literature summary for 1.10.3.2 extracted from

  • Lu, L.; Zhao, M.; Zhang, B.B.; Yu, S.Y.; Bian, X.J.; Wang, W.; Wang, Y.
    Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme (2007), Appl. Microbiol. Biotechnol., 74, 1232-1239.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol strong inhibition at 1 mM Trametes sanguinea
EDTA slight inhibition Trametes sanguinea
L-cysteine strong inhibition at 1 mM Trametes sanguinea
Sodium azide strong inhibition at 0.1 mM Trametes sanguinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 25°C Trametes sanguinea
0.091
-
 syringaldazine pH 5.0, 25°C Trametes sanguinea
0.203
-
 2,6-dimethoxyphenol pH 4.0, 25°C Trametes sanguinea

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a multicopper oxidase Trametes sanguinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61400
-
 x * 61400, SDS-PAGE Trametes sanguinea

Organism

Organism UniProt Comment Textmining
Trametes sanguinea
-
 a white rot fungus
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 3.61fold by ultrafiltration, anion exchange chromatography, and gel filtration Trametes sanguinea

Source Tissue

Source Tissue Comment Organism Textmining
additional information cultured on potato dextrose agar Trametes sanguinea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Trametes sanguinea
340.8
-
 purified enzyme Trametes sanguinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sanguinea ?
-
 ?
2,6-dimethoxyphenol + O2 preferred substrate Trametes sanguinea 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. coerulignone ?
additional information decolorization of an anthraquinone dye by the enzyme, best at pH 3.0 and 50°C, overview Trametes sanguinea ?
-
 ?
syringaldazine + O2
-
 Trametes sanguinea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61400, SDS-PAGE Trametes sanguinea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Trametes sanguinea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified enzyme, stable up to Trametes sanguinea
50
-
 purified enzyme, rapid decrease in activity Trametes sanguinea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 syringaldazine pH 5.0, 25°C Trametes sanguinea
6.91
-
 2,6-dimethoxyphenol pH 4.0, 25°C Trametes sanguinea
51.52
-
 syringaldazine pH 5.0, 25°C Trametes sanguinea
68.87
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 25°C Trametes sanguinea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 5 dependent on the substrate Trametes sanguinea

pH Range

pH Minimum pH Maximum Comment Organism
2 5 the purified enzyme is highly active within this range Trametes sanguinea