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Literature summary for 1.10.3.2 extracted from
- Studies on the Pycnoporus sanguineus CCT-4518 laccase purified by hydrophobic interaction chromatography (2007), Appl. Microbiol. Biotechnol., 75, 311-318.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 93% inhibition at 1 mM | Trametes sanguinea |  |
| HgCl2 | 46% inhibition at 51 mM | Trametes sanguinea | |
| L-cysteine | 98% inhibition at 1 mM | Trametes sanguinea | |
| additional information | no inhibition by DMSO up to 25 mM | Trametes sanguinea | |
| NaF | 53% inhibition at 0.1 mM | Trametes sanguinea | |
| NaN3 | 77% inhibition at 0.01 mM | Trametes sanguinea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
syringaldazine | pH 4.2, 50°C | Trametes sanguinea | |
| 0.058 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.2, 50°C | Trametes sanguinea | |
| 0.37 | - |
guaiacol | pH 4.2, 50°C | Trametes sanguinea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a multicopper oxidase | Trametes sanguinea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Trametes sanguinea |
| 69000 | - |
1 * 69000, SDS-PAGE | Trametes sanguinea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Trametes sanguinea | laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process | ? | - |
? | |
| additional information | Trametes sanguinea CCT-4518 | laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes sanguinea | - |
- |
- |
| Trametes sanguinea CCT-4518 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme purified 54.1fold by two steps of hydrophobic interaction chromatography | Trametes sanguinea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
purified enzyme, substrate syringaldazine | Trametes sanguinea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes sanguinea | ? | - |
? | |
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes sanguinea CCT-4518 | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | preferred substrate | Trametes sanguinea | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | i.e. coerulignone | ? | |
| 2,6-dimethoxyphenol + O2 | preferred substrate | Trametes sanguinea CCT-4518 | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | i.e. coerulignone | ? | |
| guaiacol + O2 | - |
Trametes sanguinea | ? | - |
? | |
| additional information | laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process | Trametes sanguinea | ? | - |
? | |
| additional information | substrate specificity, overview, no activity with tyrosine, phenol, vanillin, and gallic acid | Trametes sanguinea | ? | - |
? | |
| additional information | laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process | Trametes sanguinea CCT-4518 | ? | - |
? | |
| additional information | substrate specificity, overview, no activity with tyrosine, phenol, vanillin, and gallic acid | Trametes sanguinea CCT-4518 | ? | - |
? | |
| syringaldazine + O2 | - |
Trametes sanguinea | ? | - |
? | |
| syringaldazine + O2 | - |
Trametes sanguinea CCT-4518 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 69000, SDS-PAGE | Trametes sanguinea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
with substrate syringaldazine | Trametes sanguinea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 55 | - |
Trametes sanguinea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, 93% remaining activity after 24 h, 76% remaining activity after 48 h | Trametes sanguinea |
| 60 | - |
purified enzyme, 2 h, 80% remaining activity | Trametes sanguinea |
| 70 | - |
purified enzyme, 2 h, 40% remaining activity | Trametes sanguinea |
| 80 | - |
purified enzyme, 2 h, 5% remaining activity, half-life at 80°C is 20 min | Trametes sanguinea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
with substrate syringaldazine | Trametes sanguinea |
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