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Literature summary for 1.10.3.2 extracted from
- Activity and stability of laccase in conjugation with chitosan (2005), Int. J. Biol. Macromol., 35, 89-95.
General Stability
| General Stability | Organism |
|---|---|
| activity and stability of laccase in conjugation with chitosan is reduced, overview | Trametes versicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chitosan | conjugated to the enzyme, with 81-93% laccase being conjugated, a moderate activity loss of 16-28% occurs in conjugation solution, a second severe activity loss of 63-78% occurs during a cycle of phase change consisting of precipitation, centrifugation and re-dissolution of the enzymechitosan conjugates, the chitosan molecular size has little effect, overview | Trametes versicolor |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0423 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | free enzyme | Trametes versicolor | |
| 0.0452 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 505 kDa | Trametes versicolor | |
| 0.0498 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 214 kDa | Trametes versicolor | |
| 0.0852 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 432 kDa | Trametes versicolor | |
| 0.095 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 131 kDa | Trametes versicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | multi-copper-containing enzyme | Trametes versicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000 | Trametes versicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes versicolor | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative activity of laccase in conjugation with chitosans of different molecular weights, overview | Trametes versicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000 | Trametes versicolor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 505 kDa | Trametes versicolor | |
| 0.08 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 214 kDa | Trametes versicolor | |
| 0.101 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 432 kDa | Trametes versicolor | |
| 0.168 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | enzyme conjugated with chitosan of 131 kDa | Trametes versicolor | |
| 0.252 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | free enzyme | Trametes versicolor | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profiles of free and chitosan-conjugated enzyme | Trametes versicolor |
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