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Literature summary for 1.10.3.2 extracted from
- Kinetics of laccase-catalysed TEMPO oxidation (2005), J. Mol. Catal. B, 37, 79-83.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 10-(3-propylsulfonate) phenoxazine | i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation | Coprinopsis cinerea |  |
| 10-(3-propylsulfonate) phenoxazine | i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation | Rhizoctonia solani | |
| 10-(3-propylsulfonate) phenoxazine | i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation | Thermothelomyces heterothallicus | |
| 10-(3-propylsulfonate) phenoxazine | i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation | Polyporus pinsitus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, laccases copper type I redox potential | Rhizoctonia solani | |
| additional information | - |
additional information | MichaelisMenten kinetics, laccases copper type I redox potential | Coprinopsis cinerea | |
| additional information | - |
additional information | MichaelisMenten kinetics, laccases copper type I redox potential | Thermothelomyces heterothallicus | |
| additional information | - |
additional information | MichaelisMenten kinetics, laccases copper type I redox potential | Polyporus pinsitus | |
| 0.0095 | - |
2,2,6,6-tetramethyl-piperidine-1-oxyl radical | pH 5.0, 25°C, recombinant enzyme | Polyporus pinsitus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | copper-containing enzyme | Coprinopsis cinerea | |
| Cu2+ | copper-containing enzyme | Rhizoctonia solani | |
| Cu2+ | copper-containing enzyme | Thermothelomyces heterothallicus | |
| Cu2+ | copper-containing enzyme | Polyporus pinsitus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | - |
- |
- |
| Polyporus pinsitus | - |
- |
- |
| Rhizoctonia solani | - |
- |
- |
| Thermothelomyces heterothallicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| further purification of the commercial preparation by anion exchange chromatography | Coprinopsis cinerea |
| further purification of the commercial preparation by anion exchange chromatography | Rhizoctonia solani |
| further purification of the commercial preparation by anion exchange chromatography | Thermothelomyces heterothallicus |
| further purification of the commercial preparation by anion exchange chromatography | Polyporus pinsitus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Coprinopsis cinerea | - |
| commercial preparation | - |
Rhizoctonia solani | - |
| commercial preparation | - |
Thermothelomyces heterothallicus | - |
| commercial preparation | - |
Polyporus pinsitus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2 | i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation | Coprinopsis cinerea | ? | - |
? | |
| 2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2 | i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation | Rhizoctonia solani | ? | - |
? | |
| 2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2 | i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation | Thermothelomyces heterothallicus | ? | - |
? | |
| 2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2 | i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation | Polyporus pinsitus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Coprinopsis cinerea |
| 25 | - |
assay at | Rhizoctonia solani |
| 25 | - |
assay at | Thermothelomyces heterothallicus |
| 25 | - |
assay at | Polyporus pinsitus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Polyporus pinsitus |
| 5.5 | - |
assay at | Coprinopsis cinerea |
| 5.5 | - |
assay at | Rhizoctonia solani |
| 5.5 | - |
assay at | Thermothelomyces heterothallicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.3 | - |
Coprinopsis cinerea |
| 4.5 | 7.3 | - |
Rhizoctonia solani |
| 4.5 | 7.3 | - |
Thermothelomyces heterothallicus |
| 4.5 | 7.3 | - |
Polyporus pinsitus |
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