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Literature summary for 1.10.3.2 extracted from

  • Madzak, C.; Mimmi, M.C.; Caminade, E.; Brault, A.; Baumberger, S.; Briozzo, P.; Mougin, C.; Jolivalt, C.
    Shifting the optimal pH of activity for a laccase from the fungus Trametes versicolor by structure-based mutagenesis (2006), Protein Eng. Des. Sel., 19, 77-84.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Yarrowia lipolytica Po1t prototrophic strain Trametes versicolor

Protein Variants

Protein Variants Comment Organism
D206A site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme Trametes versicolor
D206E site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme Trametes versicolor
D206N site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme Trametes versicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206A Trametes versicolor
0.016
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206N Trametes versicolor
0.026
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206E Trametes versicolor
0.05
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant wild-type enzyme Trametes versicolor
0.14
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206A Trametes versicolor
0.19
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant wild-type enzyme Trametes versicolor
1.63
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206N Trametes versicolor
3.28
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206E Trametes versicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ coordination of Cu1 with His458 Trametes versicolor

Organism

Organism UniProt Comment Textmining
Trametes versicolor Q96UT7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes versicolor ?
-
 ?
2,5-xylidine + O2 the enzyme also transforms the non-phenolic substrate at pH 5.0, native and recombinant enzymes, wild-type and mutant enzymes, overview Trametes versicolor ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes versicolor 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
coniferyl alcohol + O2 the enzyme transforms the non-phenolic substrate at pH 4.5 only when recombinantly expressed in the yeast Yarrowia lipolytica strain Po1t, overview Trametes versicolor ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trametes versicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206E Trametes versicolor
0.42
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206A Trametes versicolor
0.51
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant wild-type enzyme Trametes versicolor
0.66
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206N Trametes versicolor
1.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant wild-type enzyme Trametes versicolor
1.6
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206A Trametes versicolor
3.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206E Trametes versicolor
4.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206N Trametes versicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 3.4 assay at, dependent on the substrate Trametes versicolor