Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) resulting in strains AH3547 and AH3560 | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
recombinant mutant enzymes I494A and L386A at 20°C and 10.8 mg/ml or 7.9 mg/ml, respectively, from a crystallization solution containing 0.1 M sodium citrate, 8-10% PEG MME 5000 and 14% propan-2-ol at pH 5.5, X-ray diffraction structure determination and analysis at 1.6-2.9 A resolution, cryoprotection of crystals of mutants I494A and L386A by 22% ethylene glycol or 25% glycerol, respectively | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
I494A | site-directed mutagenesis at a hydrophobic residue in the vicinity of the type 1 copper site, the replacement of Ile494 by an alanine residue leads to significant changes in the enzyme, the mutant shows differences in the type 1 as well as in the type 2 copper centre compared to the wild-type enzyme | Bacillus subtilis |
L386A | the site-directed mutation of Leu386, a hydrophobic residue in the vicinity of the type 1 copper site, to an alanine residue appears to cause only very subtle alterations in the properties of the enzyme indicating minimal changes in the structure of the copper centres | Bacillus subtilis |
M502F | replacement of Met502, which is weakly co-ordinating to the T1 copper, in CotA laccase by the non-co-ordinating residues leucine and phenylalanine allows the maintenance of the T1 copper geometry while causing an increase in the redox potential | Bacillus subtilis |
M502L | replacement of Met502, which is weakly co-ordinating to the T1 copper, in CotA laccase by the non-co-ordinating residues leucine and phenylalanine allows the maintenance of the T1 copper geometry while causing an increase in the redox potential | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
syringaldazine | pH 7.0, 25°C, wild-type CotA | Bacillus subtilis | |
0.033 | - |
syringaldazine | pH 7.0, 25°C, mutant L386A | Bacillus subtilis | |
0.052 | - |
syringaldazine | pH 8.0, 25°C, mutant I494A | Bacillus subtilis | |
0.124 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, wild-type CotA | Bacillus subtilis | |
0.145 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, mutant L386A | Bacillus subtilis | |
0.227 | - |
2,6-dimethoxyphenol | pH 7.0, 25°C, wild-type CotA | Bacillus subtilis | |
0.576 | - |
2,6-dimethoxyphenol | pH 7.0, 25°C, mutant L386A | Bacillus subtilis | |
1.295 | - |
2,6-dimethoxyphenol | pH 9.0, 25°C, mutant I494A | Bacillus subtilis | |
2.027 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, mutant I494A | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper enzyme, the wild-type enzyme contains 3.7 mol Cu2+ per mol of enzyme, mutants I494A and L386A contain 4.0 mol copper per mol of enzyme. Binding structures in wild-type and mutant enzymes I494A and L386A, analysis of crystal structures and simulation, overview | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
strains AH3517 containing pLOM10, AH3547 and AH3560 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus subtilis | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Bacillus subtilis | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CotA | - |
Bacillus subtilis |
More | laccases are members of the MCO, multicopper oxidase, family of enzymes | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | 2,6-dimethoxyphenol | pH 7.0, 25°C, wild-type CotA | Bacillus subtilis | |
4.5 | - |
2,6-dimethoxyphenol | pH 9.0, 25°C, mutant I494A | Bacillus subtilis | |
7.2 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, mutant I494A | Bacillus subtilis | |
9 | - |
syringaldazine | pH 8.0, 25°C, mutant I494A | Bacillus subtilis | |
13 | - |
syringaldazine | pH 7.0, 25°C, mutant L386A | Bacillus subtilis | |
17 | - |
2,6-dimethoxyphenol | pH 7.0, 25°C, mutant L386A | Bacillus subtilis | |
52 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, mutant L386A | Bacillus subtilis | |
80 | - |
syringaldazine | pH 7.0, 25°C, wild-type CotA | Bacillus subtilis | |
322 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, wild-type CotA | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, wild-type enzyme and mutant enzymes | Bacillus subtilis |
7 | - |
substrate 2,6-dimethoxyphenol, assay at for the wild-type enzyme and the L386A mutant | Bacillus subtilis |
7.6 | - |
redox titration assay at | Bacillus subtilis |
8 | 9 | for the I494A mutant | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profiles of wild-type and mutant enzymes | Bacillus subtilis |