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Literature summary for 1.10.3.2 extracted from

  • Junghanns, C.; Parra, R.; Keshavarz, T.; Schlosser, D.
    Towards higher laccase activities produced by aquatic ascomycetous fungi through combination of elicitors and an alternative substrate (2008), Eng. Life Sci., 8, 277-285.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Cu2+ activates, increases the enzyme activity best at 0.05 mM in the culture medium Coniothyrium sp.

Application

Application Comment Organism
additional information laccases are versatile biocatalysts with various potential biotechnological applications, e.g. the treatment of industrial waste waters, the detoxification of environmental pollutants, or the functionalization of renewable polymeric materials Phoma sp.
additional information laccases are versatile biocatalysts with various potential biotechnological applications, e.g. the treatment of industrial waste waters, the detoxification of environmental pollutants, or the functionalization of renewable polymeric materials Coniothyrium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates, increases the enzyme activity best at 0.25 mM in the culture medium Phoma sp.

Organism

Organism UniProt Comment Textmining
Coniothyrium sp.
-
isolated from surface water bodies
-
Coniothyrium sp. Kl-S5
-
isolated from surface water bodies
-
Phoma sp.
-
isolated from surface water bodies
-
Phoma sp. UHH 5-1-03 / DSM 22425
-
isolated from surface water bodies
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of liquid culture conditions for high enzyme expression rates, overview Phoma sp.
-
additional information optimization of liquid culture conditions for high enzyme expression rates, overview Coniothyrium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30 35 native enzyme in optimized culture Coniothyrium sp.
6322
-
native enzyme in optimized culture Phoma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Phoma sp. ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Coniothyrium sp. ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Coniothyrium sp. Kl-S5 ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
assay at Phoma sp.
4
-
assay at Coniothyrium sp.