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Literature summary for 1.10.3.2 extracted from

  • Vite-Vallejo, O.; Palomares, L.; Dantan-Gonzalez, E.; Ayala-Castro, H.; Marti­nez-Anaya, C.; Valderrama, B.; Folch-Mallol, J.
    The role of N-glycosylation on the enzymatic activity of a Pycnoporus sanguineus laccase (2009), Enzyme Microb. Technol., 45, 233-239.
No PubMed abstract available
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Trametes sanguinea

Inhibitors

Inhibitors Comment Organism Structure
Urea inactivation at 5 M, 50% inhibition at about 2 M Trametes sanguinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 2-dianisidine pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea
0.044
-
 2-dianisidine pH 4.5, 22°C, native enzyme Trametes sanguinea
0.102
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea
0.102
-
 syringaldazine pH 4.5, 22°C, native enzyme Trametes sanguinea
0.106
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.5, 22°C, native enzyme Trametes sanguinea
0.156
-
 syringaldazine pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea
0.25
-
 guaiacol pH 4.5, 22°C, native enzyme Trametes sanguinea
0.367
-
 guaiacol pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme contains 4 copper molecules per enzyme molecule, structure overview Trametes sanguinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, SDS-PAGE Trametes sanguinea

Organism

Organism UniProt Comment Textmining
Trametes sanguinea C1JCL7
-
-
Trametes sanguinea CeIBMD001 C1JCL7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is N-glycosylated, five possible N-glycosylation sites at N74, N237, N311, N354, and N362, profile, overview Trametes sanguinea

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, cation exchange chromatography, ultrafiltration, and gel filtration to homogeneity Trametes sanguinea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trametes sanguinea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69
-
 purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes sanguinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes sanguinea ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes sanguinea CeIBMD001 ?
-
 ?
2-dianisidine + O2
-
 Trametes sanguinea ?
-
 ?
2-dianisidine + O2
-
 Trametes sanguinea CeIBMD001 ?
-
 ?
guaiacol + O2
-
 Trametes sanguinea ?
-
 ?
guaiacol + O2
-
 Trametes sanguinea CeIBMD001 ?
-
 ?
syringaldazine + O2
-
 Trametes sanguinea ?
-
 ?
syringaldazine + O2
-
 Trametes sanguinea CeIBMD001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Trametes sanguinea
More three-dimensional structure modelling, overview Trametes sanguinea

Synonyms

Synonyms Comment Organism
More the enzyme is a blue laccase Trametes sanguinea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Trametes sanguinea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 activity range Trametes sanguinea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 temperature stability profiles of native and deglycosylated enzymes, overview Trametes sanguinea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.05
-
 guaiacol pH 4.5, 22°C, native enzyme Trametes sanguinea
3
-
 guaiacol pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea
4.9
-
 2-dianisidine pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea
9.4
-
 2-dianisidine pH 4.5, 22°C, native enzyme Trametes sanguinea
39.9
-
 syringaldazine pH 4.5, 22°C, native enzyme Trametes sanguinea
48.6
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea
59.3
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.5, 22°C, native enzyme Trametes sanguinea
65.8
-
 syringaldazine pH 4.5, 22°C, deglycosylated enzyme Trametes sanguinea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes sanguinea

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
 Trametes sanguinea

pI Value

Organism Comment pI Value Maximum pI Value
Trametes sanguinea isoelectric focussing
-
 4.5