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Literature summary for 1.10.3.2 extracted from
- Characterization of endogenous and recombinant forms of laccase-2, a multicopper oxidase from the tobacco hornworm, Manduca sexta (2009), Insect Biochem. Mol. Biol., 39, 596-606.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of full-length and amino-terminally truncated laccase-2, both showing lower activity than the native enzyme | Manduca sexta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme shows typical MichaelisMenten kinetics, overview | Manduca sexta | |
| 0.43 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant N-terminally truncated enzyme | Manduca sexta |  |
| 0.46 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
| 0.47 | - |
N-beta-alanyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
| 0.63 | - |
N-acetyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
| 1.9 | - |
N-beta-alanyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a multicopper oxidase | Manduca sexta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE | Manduca sexta |
| 90000 | - |
x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE | Manduca sexta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Manduca sexta | Q8I8Y0 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Manduca sexta |
| additional information | laccase-2 is expressed in an active form rather than as a zymogen | Manduca sexta |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from the pharate pupal cuticle to homogeneity | Manduca sexta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | laccase-2 is expressed in an active form rather than as a zymogen | Manduca sexta | - |
| pharate pupal cuticle | - |
Manduca sexta | - |
| pupal wing cuticle | - |
Manduca sexta | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.017 | - |
native enzyme in pharate pupa and pupal wing | Manduca sexta |
| 0.41 | - |
purified native enzyme, substrate syringaldazine | Manduca sexta |
| 4.02 | - |
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Manduca sexta |
| 4.18 | - |
purified native enzyme, substrate N-beta-alanyldopamine-alanyldopamine | Manduca sexta |
| 5.01 | - |
purified native enzyme, substrate N-acetyldopamine | Manduca sexta |
| 10.06 | - |
purified native enzyme, substrate hydrochinone | Manduca sexta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Manduca sexta | ? | - |
? | |
| hydroquinone + O2 | - |
Manduca sexta | ? | - |
? | |
| additional information | no activity with L-tyrosine | Manduca sexta | ? | - |
? | |
| N-acetyldopamine + O2 | - |
Manduca sexta | ? | - |
? | |
| N-beta-alanyldopamine + O2 | - |
Manduca sexta | ? | - |
? | |
| syringaldazine + O2 | - |
Manduca sexta | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE | Manduca sexta |
| More | peptide mass fingerprinting analysis, mass spectrometry, overview | Manduca sexta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| laccase-2 | - |
Manduca sexta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.33 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant N-terminally truncated enzyme | Manduca sexta | |
| 1.67 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
| 3 | - |
N-beta-alanyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
| 3.33 | - |
N-beta-alanyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
| 4.83 | - |
N-acetyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Manduca sexta |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH profiles of native and recombinant enzymes, overview | Manduca sexta |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in cuticle sclerotization | Manduca sexta |
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