Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Galai, S.; Limam, F.; Marzouki, M.N.
    A new Stenotrophomonas maltophilia strain producing laccase. Use in decolorization of synthetics dyes (2008), Appl. Biochem. Biotechnol., 158, 416-431.
    View publication on PubMed
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
Triton-X-100 activated by addition of 0.1% (v/v) Triton-X-100 Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, complete inhibition Stenotrophomonas maltophilia
Fe2+ 10 mM, about 75% inhibition Stenotrophomonas maltophilia
Mn2+ 10 mM, about 30% inhibition Stenotrophomonas maltophilia
Sodium azide 5 mM, 40% inhibition Stenotrophomonas maltophilia
Urea 0.4 mM, complete inhibition Stenotrophomonas maltophilia
Zn2+ 10 mM, about 30% inhibition Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 catechol 40°C, pH 4.5 Stenotrophomonas maltophilia
0.05
-
 syringaldazine 40°C, pH 4.5 Stenotrophomonas maltophilia
0.7
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) 40°C, pH 4.5 Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, weak activation Stenotrophomonas maltophilia
Cu2+ laccase activity is increased 2.6-fold by the addition of 10 mM copper sulfate Stenotrophomonas maltophilia
Mg2+ 10 mM, weak activation Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia AAP56
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
 Stenotrophomonas maltophilia 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Stenotrophomonas maltophilia AAP56 2 1,2-benzoquinone + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Stenotrophomonas maltophilia AAP56 ?
-
 ?
syringaldazine + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
syringaldazine + O2
-
 Stenotrophomonas maltophilia AAP56 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate: syringaldazine Stenotrophomonas maltophilia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 30% of maximal activity, 70°C: about 50% of maximal activity Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, 52% loss of activity Stenotrophomonas maltophilia
50
-
 30 min, 75% loss of activity Stenotrophomonas maltophilia
70
-
 30 min, complete inactivation Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in 50 mM phosphate buffer with syringaldazine as substrate Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 1 h, stable Stenotrophomonas maltophilia