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Literature summary for 1.10.3.2 extracted from

  • Chairin, T.; Nitheranont, T.; Watanabe, A.; Asada, Y.; Khanongnuch, C.; Lumyong, S.
    Purification and characterization of the extracellular laccase produced by Trametes polyzona WR710-1 under solid-state fermentation (2013), J. Basic Microbiol., 54, 35-43.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 14% inhibition at 5 mM Trametes polyzona
Co2+ 28% inhibition at 5 mM Trametes polyzona
Cr3+ 23% inhibition at 5 mM Trametes polyzona
Cu2+ 19% inhibition at 5 mM Trametes polyzona
DTT complete inhibition at 1 mM Trametes polyzona
Fe2+ 95% inhibition at 5 mM Trametes polyzona
L-cysteine complete inhibition at 10 mM Trametes polyzona
Mg2+ 6% inhibition at 5 mM Trametes polyzona
NaCl 50% inhibition at 20 mM, the inhibitory effect of chloride ions is reversible Trametes polyzona
NaN3 complete inhibition at 0.1 mM Trametes polyzona
Zn2+ 19% inhibition at 5 mM Trametes polyzona

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, temperature not specified in the publication Trametes polyzona
0.503
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication Trametes polyzona
1.89
-
 guaiacol pH 5.0, temperature not specified in the publication Trametes polyzona
4.08
-
 catechol pH 5.0, temperature not specified in the publication Trametes polyzona
6.28
-
 L-Dopa pH 5.0, temperature not specified in the publication Trametes polyzona

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trametes polyzona
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 native enzyme, gel filtration Trametes polyzona
71000
-
 1 * 71000, SDS-PAGE Trametes polyzona

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 catechol + O2 Trametes polyzona
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
4 catechol + O2 Trametes polyzona WR710-1
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Trametes polyzona
-
-
-
Trametes polyzona WR710-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 45fold from solid-state fermentation on peel from Citrus reticulata to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, ultrafiltration, and gel filtration Trametes polyzona

Source Tissue

Source Tissue Comment Organism Textmining
additional information laccase from Trametes polyzona WR710-1 is produced under solid-state fermentation using the peel from the Tangerine orange (Citrus reticulata Blanco) as substrate Trametes polyzona
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.8
-
 purified native extracellular enzyme, pH 5.0, temperature not specified in the publication, substrate 2,6-dimethoxyphenol Trametes polyzona

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes polyzona ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes polyzona WR710-1 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes polyzona ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes polyzona WR710-1 ?
-
 ?
4 catechol + O2
-
 Trametes polyzona 4 1,2-benzoquinone + 2 H2O
-
 ?
4 catechol + O2
-
 Trametes polyzona WR710-1 4 1,2-benzoquinone + 2 H2O
-
 ?
guaiacol + O2
-
 Trametes polyzona ?
-
 ?
guaiacol + O2
-
 Trametes polyzona WR710-1 ?
-
 ?
L-DOPA + O2
-
 Trametes polyzona ?
-
 ?
L-DOPA + O2
-
 Trametes polyzona WR710-1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE Trametes polyzona

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified native enzyme, 60 min, completely stable up to Trametes polyzona
50
-
 purified native enzyme, 60 min, 10% loss of activity Trametes polyzona
70
-
 purified native enzyme, 60 min, inactivation Trametes polyzona

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.2
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication Trametes polyzona
83.2
-
 L-Dopa pH 5.0, temperature not specified in the publication Trametes polyzona
92.2
-
 guaiacol pH 5.0, temperature not specified in the publication Trametes polyzona
125
-
 catechol pH 5.0, temperature not specified in the publication Trametes polyzona
594
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, temperature not specified in the publication Trametes polyzona

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Trametes polyzona
4
-
 with substrates L-DOPA, guaiacol, and catechol Trametes polyzona
5
-
 with substrate 2,6-dimethoxyphenol Trametes polyzona

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable within this range Trametes polyzona

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.3
-
 L-Dopa pH 5.0, temperature not specified in the publication Trametes polyzona
30.6
-
 catechol pH 5.0, temperature not specified in the publication Trametes polyzona
48.7
-
 guaiacol pH 5.0, temperature not specified in the publication Trametes polyzona
75.9
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication Trametes polyzona
3960
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, temperature not specified in the publication Trametes polyzona