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Literature summary for 1.10.3.2 extracted from
- Recombinant laccase: I. Enzyme cloning and characterization (2013), J. Cell. Biochem., 114, 599-605.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Laccase-encoding cDNA, expression and procedure optimization of a recombinant His-tagged laccase expressed in supercompetent Escherichia coli strain B F-dcm ompT hsdS (rB-, mB-) gal lambda (DE3) | Rigidoporus microporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rigidoporus microporus | - |
formerly Rigidoporus microporus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-taagged enzyme from supercompetent Escherichia coli cells, procedure optimization including affinity chromatography and gel filtration | Rigidoporus microporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| syringaldazine + O2 | - |
Rigidoporus microporus | ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rigidoporus microporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Rigidoporus microporus |
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Release 2023.1 (January 2023)
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