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Literature summary for 1.10.3.2 extracted from

  • Ben Younes, S.; Sayadi, S.
    Purification and characterization of a novel trimeric and thermotolerant laccase produced from the ascomycete Scytalidium thermophilum strain (2011), J. Mol. Catal. B, 73, 35-42.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
DTT
-
Mycothermus thermophilus
EDTA
-
Mycothermus thermophilus
Fe2+ inhibits laccase activity completely at 10 mM Mycothermus thermophilus
additional information poor inhibition by NaF and NaBr Mycothermus thermophilus
NaN3
-
Mycothermus thermophilus
SDS
-
Mycothermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, overview Mycothermus thermophilus
0.026
-
2,6-dimethoxyphenol pH 5.0, 22-25°C Mycothermus thermophilus
0.036
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22-25°C Mycothermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Mycothermus thermophilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information laccase activity remains stable for 24 h against all the ions tested, i.e. Cu2+, Co2+, Pb2+, Ca2+, Cd2+, Mg2+, Mn2+, Fe2+, Ni2+, F-, Zn2+, and Li+, at 10-100 mM Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
gel filtration Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus FJ560721 isolated from locally prepared compost in the north of Tunisia
-
Mycothermus thermophilus ST26 FJ560721 isolated from locally prepared compost in the north of Tunisia
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 8fold by two teps of anion exchange chromatography, and gel filtration to homogeneity Mycothermus thermophilus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Mycothermus thermophilus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
139.4
-
purified native enzyme, pH 5.0, 22-25°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Mycothermus thermophilus ?
-
?
2,6-dimethoxyphenol + O2
-
Mycothermus thermophilus ?
-
?
2,6-dimethoxyphenol + O2
-
Mycothermus thermophilus ST26 ?
-
?
4 catechol + O2
-
Mycothermus thermophilus 4 1,2-benzoquinone + 2 H2O
-
?
guaiacol + O2
-
Mycothermus thermophilus ?
-
?
guaiacol + O2
-
Mycothermus thermophilus ST26 ?
-
?
hydroquinone + O2
-
Mycothermus thermophilus ?
-
?
hydroquinone + O2
-
Mycothermus thermophilus ST26 ?
-
?
additional information substrate specificity of purified enzyme, overview. No activity with ferulic acid Mycothermus thermophilus ?
-
?
additional information substrate specificity of purified enzyme, overview. No activity with ferulic acid Mycothermus thermophilus ST26 ?
-
?
pyrogallol + O2
-
Mycothermus thermophilus ?
-
?
pyrogallol + O2
-
Mycothermus thermophilus ST26 ?
-
?

Subunits

Subunits Comment Organism
homotrimer 3 x 28000, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
with substrate 2,6-dimethoxyphenol Mycothermus thermophilus
80
-
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Mycothermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 85 activity range Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
the purified native enzyme retains 50% of its activity after 120 min of incubation Mycothermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22-25°C Mycothermus thermophilus
0.35
-
2,6-dimethoxyphenol pH 5.0, 22-25°C Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
with substrates 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 2,6-dimethoxyphenol Mycothermus thermophilus
6
-
with substrates guaiacol and hydroquinone Mycothermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.375
-
2,6-dimethoxyphenol pH 5.0, 22-25°C Mycothermus thermophilus
5.05
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22-25°C Mycothermus thermophilus