Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Mycothermus thermophilus | |
EDTA | - |
Mycothermus thermophilus | |
Fe2+ | inhibits laccase activity completely at 10 mM | Mycothermus thermophilus | |
additional information | poor inhibition by NaF and NaBr | Mycothermus thermophilus | |
NaN3 | - |
Mycothermus thermophilus | |
SDS | - |
Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Mycothermus thermophilus | |
0.026 | - |
2,6-dimethoxyphenol | pH 5.0, 22-25°C | Mycothermus thermophilus | |
0.036 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22-25°C | Mycothermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Mycothermus thermophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | laccase activity remains stable for 24 h against all the ions tested, i.e. Cu2+, Co2+, Pb2+, Ca2+, Cd2+, Mg2+, Mn2+, Fe2+, Ni2+, F-, Zn2+, and Li+, at 10-100 mM | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
gel filtration | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | FJ560721 | isolated from locally prepared compost in the north of Tunisia | - |
Mycothermus thermophilus ST26 | FJ560721 | isolated from locally prepared compost in the north of Tunisia | - |
Purification (Comment) | Organism |
---|---|
native enzyme 8fold by two teps of anion exchange chromatography, and gel filtration to homogeneity | Mycothermus thermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Mycothermus thermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
139.4 | - |
purified native enzyme, pH 5.0, 22-25°C | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Mycothermus thermophilus | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Mycothermus thermophilus | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Mycothermus thermophilus ST26 | ? | - |
? | |
4 catechol + O2 | - |
Mycothermus thermophilus | 4 1,2-benzoquinone + 2 H2O | - |
? | |
guaiacol + O2 | - |
Mycothermus thermophilus | ? | - |
? | |
guaiacol + O2 | - |
Mycothermus thermophilus ST26 | ? | - |
? | |
hydroquinone + O2 | - |
Mycothermus thermophilus | ? | - |
? | |
hydroquinone + O2 | - |
Mycothermus thermophilus ST26 | ? | - |
? | |
additional information | substrate specificity of purified enzyme, overview. No activity with ferulic acid | Mycothermus thermophilus | ? | - |
? | |
additional information | substrate specificity of purified enzyme, overview. No activity with ferulic acid | Mycothermus thermophilus ST26 | ? | - |
? | |
pyrogallol + O2 | - |
Mycothermus thermophilus | ? | - |
? | |
pyrogallol + O2 | - |
Mycothermus thermophilus ST26 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 x 28000, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
with substrate 2,6-dimethoxyphenol | Mycothermus thermophilus |
80 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Mycothermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | activity range | Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the purified native enzyme retains 50% of its activity after 120 min of incubation | Mycothermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22-25°C | Mycothermus thermophilus | |
0.35 | - |
2,6-dimethoxyphenol | pH 5.0, 22-25°C | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
with substrates 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 2,6-dimethoxyphenol | Mycothermus thermophilus |
6 | - |
with substrates guaiacol and hydroquinone | Mycothermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.375 | - |
2,6-dimethoxyphenol | pH 5.0, 22-25°C | Mycothermus thermophilus | |
5.05 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22-25°C | Mycothermus thermophilus |