Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Bao, S.; Teng, Z.; Ding, S.
    Heterologous expression and characterization of a novel laccase isoenzyme with dyes decolorization potential from Coprinus comatus (2013), Mol. Biol. Rep., 40, 1927-1936.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lac2, DNA and amino acid sequence determination and analysis Coprinus comatus
genes lac1 and lac2, DNA and amino acid sequence determination and analysis, expression of Lac1 in Pichia pastoris Coprinus comatus

Inhibitors

Inhibitors Comment Organism Structure
DTT complete inhibition at 5 mM Coprinus comatus
NaN3 complete inhibition at 5 mM Coprinus comatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
0.034
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
4.317
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
7.611
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ slightly activating Coprinus comatus
K+ slightly activating Coprinus comatus
NH4+ slightly activating Coprinus comatus

Organism

Organism UniProt Comment Textmining
Coprinus comatus H9C4A2 lac2; gene lac2
-
Coprinus comatus H9C4A3 lac1; genes lac1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Lac1 from Pichia pastoris by ultrafiltration, anion exchange chromatography, ultrafiltration, and gel filtration Coprinus comatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Coprinus comatus ?
-
?
2,6-dimethylphenol + O2
-
Coprinus comatus ?
-
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Coprinus comatus ?
-
?
4 2,6-dimethylphenol + O2
-
Coprinus comatus ?
-
?
4 guaiacol + O2
-
Coprinus comatus ?
-
?
4 syringaldazine + O2
-
Coprinus comatus ?
-
?
guaiacol + O2
-
Coprinus comatus ?
-
?
additional information the enzyme shows dye decolorization potential, overview Coprinus comatus ?
-
?
syringaldazine + O2
-
Coprinus comatus ?
-
?

Synonyms

Synonyms Comment Organism
LAC1
-
Coprinus comatus
Lac2
-
Coprinus comatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
with substrates syringaldazine, recombinant Lac1 Coprinus comatus
55
-
with substrates guaiacol, recombinant Lac1 Coprinus comatus
65
-
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1 Coprinus comatus
70
-
with substrate 2,6-dimethylphenol, recombinant Lac1 Coprinus comatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.15
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
7.67
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
15.93
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1 Coprinus comatus
5.5
-
with substrate 2,6-dimethylphenol, recombinant Lac1 Coprinus comatus
6
-
with substrates guaiacol and syringaldazine, recombinant Lac1 Coprinus comatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.78
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
42.55
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
465.8
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus