Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | - |
Shiraia sp. SUPER-H168 | |
Ag+ | 8.13% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
DTT | 43% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
EDTA | 16% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
EGTA | 22% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
ethanol | - |
Shiraia sp. SUPER-H168 | |
Fe2+ | complete inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
Fe3+ | 50% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
glycerol | - |
Shiraia sp. SUPER-H168 | |
isoamyl alcohol | - |
Shiraia sp. SUPER-H168 | |
Isopropyl alcohol | - |
Shiraia sp. SUPER-H168 | |
L-cysteine | 44% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
methanol | - |
Shiraia sp. SUPER-H168 | |
n-heptane | - |
Shiraia sp. SUPER-H168 | |
n-hexane | - |
Shiraia sp. SUPER-H168 | |
NaF | 47% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
NaN3 | 7.86% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
SDS | 10.2% inhibition at 10 mM | Shiraia sp. SUPER-H168 | |
tertiary butanol | - |
Shiraia sp. SUPER-H168 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
syringaldazine | pH 6.0, 50°C | Shiraia sp. SUPER-H168 | |
0.19 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 50°C | Shiraia sp. SUPER-H168 | |
0.37 | - |
2,6-dimethoxyphenol | pH 4.0, 50°C | Shiraia sp. SUPER-H168 | |
0.38 | - |
guaiacol | pH 5.5, 50°C | Shiraia sp. SUPER-H168 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor effect on enzyme activity by K+, Ca2+, Cu2+, Mg2+, Mn2+, Zn2+, and Ba2+ at 10 mM | Shiraia sp. SUPER-H168 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 70780, mass spectrometry, x * 69000, SDS-PAGE | Shiraia sp. SUPER-H168 |
70780 | - |
x * 70780, mass spectrometry, x * 69000, SDS-PAGE | Shiraia sp. SUPER-H168 |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
Ethanol | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
glycer0l | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
isoamyl alcohol | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
isopropyl alcohol | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
Methanol | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
n-heptane | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
n-hexane | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
tert-Butanol | slight loss of activity at 10% v/v | Shiraia sp. SUPER-H168 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shiraia sp. SUPER-H168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 2.14fold by cation exchange chromatography and gel filtration | Shiraia sp. SUPER-H168 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
417.7 | - |
purified enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 50°C | Shiraia sp. SUPER-H168 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Shiraia sp. SUPER-H168 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Shiraia sp. SUPER-H168 | ? | - |
? | |
guaiacol + O2 | - |
Shiraia sp. SUPER-H168 | ? | - |
? | |
additional information | approximately 66% of the Acid Blue 129 at 100 mg/l is decolorized by 2.5 U of the purified laccase after a 120 min incubation at 50°C, Acid Red 1 at 20 mg/l and Reactive Black 5 at 50 mg/l are decolorized by the purified laccase after the addition of Acid Blue 129 at 100 mg/l. | Shiraia sp. SUPER-H168 | ? | - |
? | |
syringaldazine + O2 | - |
Shiraia sp. SUPER-H168 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70780, mass spectrometry, x * 69000, SDS-PAGE | Shiraia sp. SUPER-H168 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
with substrates 2,6-dimethoxyphenol, syringaldazine, and guaiacol | Shiraia sp. SUPER-H168 |
60 | - |
with substrate 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) | Shiraia sp. SUPER-H168 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.44 | - |
guaiacol | pH 5.5, 50°C | Shiraia sp. SUPER-H168 | |
938.1 | - |
syringaldazine | pH 6.0, 50°C | Shiraia sp. SUPER-H168 | |
1476 | - |
2,6-dimethoxyphenol | pH 4.0, 50°C | Shiraia sp. SUPER-H168 | |
7109 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 50°C | Shiraia sp. SUPER-H168 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
with substrate 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) | Shiraia sp. SUPER-H168 |
4 | - |
with substrate 2,6-dimethoxyphenol | Shiraia sp. SUPER-H168 |
5.5 | - |
with substrate guaiacol | Shiraia sp. SUPER-H168 |
6 | - |
with substrate syringaldazine | Shiraia sp. SUPER-H168 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | purified enzyme, the enzyme retains 80% of its initial activity after 96 h | Shiraia sp. SUPER-H168 |