Cloned (Comment) | Organism |
---|---|
gene lcc3, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Cerrena sp. WR1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 50°C | Cerrena sp. WR1 | |
0.393 | - |
2,6-dimethoxyphenol | pH 3.0, 50°C | Cerrena sp. WR1 | |
0.849 | - |
guaiacol | pH 3.0, 50°C | Cerrena sp. WR1 | |
0.881 | - |
catechol | pH 3.0, 50°C | Cerrena sp. WR1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Cerrena sp. WR1 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE | Cerrena sp. WR1 |
64100 | - |
x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE | Cerrena sp. WR1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 catechol + O2 | Cerrena sp. WR1 | - |
4 1,2-benzoquinone + 2 H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitril | 77.5% activity remaining after 14 days in 25% | Cerrena sp. WR1 |
dimethyl sulfoxide | 55.8% activity remaining after 14 days in 25% | Cerrena sp. WR1 |
Ethanol | 84.8% activity remaining after 14 days in 25% | Cerrena sp. WR1 |
Methanol | 74.6% activity remaining after 14 days in 25% | Cerrena sp. WR1 |
N,N-dimethylformamide | 81.2% activity remaining after 14 days in 25% | Cerrena sp. WR1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena sp. WR1 | E7BLR0 | gene lcc3 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | laccase Lcc3 is N-linked glycosylated, deglycosylation by peptide N-glycosidase F, the enzyme contains six potential N-glycosylation sites, i.e. N35, N236, N272, N311, N453 and N489, with a conserved sequence of NxS/T, ire found in the deduced Lcc3 amino acid sequence | Cerrena sp. WR1 |
Purification (Comment) | Organism |
---|---|
native enzyme 24.9fold from strain WR1, by two steps of anion exchange chromatography to over 96% homogeneity | Cerrena sp. WR1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
144.3 | - |
specific activity of the crude laccase broth, pH 3.0, 50°C | Cerrena sp. WR1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,6-dimethoxyphenol + O2 | - |
Cerrena sp. WR1 | ? | - |
? | |
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Cerrena sp. WR1 | ? | - |
? | |
4 catechol + O2 | - |
Cerrena sp. WR1 | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 guaiacol + O2 | - |
Cerrena sp. WR1 | ? | - |
? | |
additional information | the enzyme efficiently performs dye decoloration of anthraquinone dye RBBR | Cerrena sp. WR1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE | Cerrena sp. WR1 |
Synonyms | Comment | Organism |
---|---|---|
Lcc3 | - |
Cerrena sp. WR1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cerrena sp. WR1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
Tm of Lcc3 is determined at 73.9°C and it shows a t1/2 of 120 min at 50°C | Cerrena sp. WR1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
109.2 | - |
2,6-dimethoxyphenol | pH 3.0, 50°C | Cerrena sp. WR1 | |
115.5 | - |
catechol | pH 3.0, 50°C | Cerrena sp. WR1 | |
147.9 | - |
guaiacol | pH 3.0, 50°C | Cerrena sp. WR1 | |
934.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 50°C | Cerrena sp. WR1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena sp. WR1 |
3.5 | - |
with substrate 2,6-dimethoxyphenol | Cerrena sp. WR1 |
4.5 | - |
with substrate guaiacol | Cerrena sp. WR1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
loss of 50% activity after 43 h, 25°C | Cerrena sp. WR1 |
4 | - |
loss of 50% activity after 82 h, 25°C | Cerrena sp. WR1 |
5 | - |
loss of 20% activity after 100 h, 25°C | Cerrena sp. WR1 |
6 | 10 | no loss of activity after 100 h, 25°C | Cerrena sp. WR1 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the blue multicopper oxidase superfamily | Cerrena sp. WR1 |