Application | Comment | Organism |
---|---|---|
degradation | cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Due to phototrophic mode of nutrition, short generation time and easy mass cultivation, Spirulina platensis laccase appears as good candidate for laccase production. The high yield of laccase in short production period are profitable for its industrial application. Pure Spirulina platensis laccase alone can efficiently decolorized anthraquinonic dye Reactive Blue 4 without any mediators which makes it cost effective and suitable candidate for decolorization of synthetic dyes and help in waste water treatment | Arthrospira platensis |
environmental protection | cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Due to phototrophic mode of nutrition, short generation time and easy mass cultivation, Spirulina platensis laccase appears as good candidate for laccase production. The high yield of laccase in short production period are profitable for its industrial application. Pure Spirulina platensis laccase alone can efficiently decolorized anthraquinonic dye Reactive Blue 4 without any mediators which makes it cost effective and suitable candidate for decolorization of synthetic dyes and help in waste water treatment | Arthrospira platensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 79%% inhibition | Arthrospira platensis | |
EDTA | 1 mM, 31% inhibition | Arthrospira platensis | |
Hg2+ | 1 mM, 4% inhibition | Arthrospira platensis | |
L-cysteine | 1 mM, 95% inhibition | Arthrospira platensis | |
Mg2+ | 1 mM, 88% inhibition | Arthrospira platensis | |
Sodium azide | 1 mM, 98% inhibition | Arthrospira platensis | |
thioglycolic acid | 1 mM, 82% inhibition | Arthrospira platensis | |
Thiourea | 1 mM, 60% inhibition | Arthrospira platensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Arthrospira platensis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, enhances activity to 137% of control | Arthrospira platensis | |
Mn2+ | 1 mM, enhances activity to 103% of control | Arthrospira platensis | |
Zn2+ | 1 mM, enhances activity to 120% of control | Arthrospira platensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
non-denaturing PAGE | Arthrospira platensis |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 10% (v/v), 15 min, 91.8% activity compared to control | Arthrospira platensis |
dimethylformamide | 10% (v/v), 15 min, 58.8% activity compared to control | Arthrospira platensis |
DMSO | 10% (v/v), 15 min, 42.3% activity compared to control | Arthrospira platensis |
Ethanol | 10% (v/v), 15 min, 96.36% activity compared to control | Arthrospira platensis |
Methanol | 10% (v/v), 15 min, 82.5% activity compared to control | Arthrospira platensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrospira platensis | - |
- |
- |
Arthrospira platensis CFTRI | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrospira platensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Arthrospira platensis | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Arthrospira platensis CFTRI | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Arthrospira platensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Arthrospira platensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 50% of maximal activity, 60°C: about 55% of maximal activioty, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Arthrospira platensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
60 min, enzyme retains 100% of its activity | Arthrospira platensis |
40 | - |
60 min, enzyme retains 97% of its activity | Arthrospira platensis |
50 | - |
30 min, enzyme retains 85.4% of its activity. 90 min, enzyme retains 80% of its activity | Arthrospira platensis |
60 | - |
30 min, enzyme retains 54.0% of its activity. 90 min, enzyme retains 48% of its activity | Arthrospira platensis |
70 | - |
30 min, enzyme retains 15.0% of its activity. 90 min, enzyme retains 10% of its activity. 180 min, complete inactivation | Arthrospira platensis |
80 | - |
90 min, complete inactivation | Arthrospira platensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Arthrospira platensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 5 | pH 2.0: about 75% of maximal activity, pH 5.0: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Arthrospira platensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
1 h, enzyme retains 100% of its activity | Arthrospira platensis |