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Literature summary for 1.10.3.2 extracted from

  • Gu, C.; Zheng, F.; Long, L.; Wang, J.; Ding, S.
    Engineering the expression and characterization of two novel laccase isoenzymes from Coprinus comatus in Pichia pastoris by fusing an additional ten amino acids tag at N-terminus (2014), PLoS ONE, 9, e93912 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in in Pichia pastoris by fusing an additional ten amino acids (Thr-Pro-Phe-Pro-Pro-Phe-Asn-Thr-Asn-Ser) tag at N-terminus Coprinus comatus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 0.1 mM, complete inactivation; 0.1 mM, complete inactivation Coprinus comatus
EDTA 50 mM, 70% loss of activity, laccase 3; 50 mM, 80% loss of activity, laccase 4 Coprinus comatus
additional information isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS Coprinus comatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.087
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
0.136
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
0.204
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
0.205
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
0.455
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.114
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.374
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
7.131
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzyme Lac4 Coprinus comatus
additional information metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzymes Lac3 and Lac4 Coprinus comatus

Organism

Organism UniProt Comment Textmining
Coprinus comatus X5JA14 isoenzyme laccase 3
-
Coprinus comatus X5JAK9 isoenzyme laccase 4
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein when expressed in Pichia pastoris, the recombinant laccase is hyperglycosylated Coprinus comatus

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Coprinus comatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinus comatus ?
-
 ?
2,6-dimethylphenol + O2
-
 Coprinus comatus ?
-
 ?
guaiacol + O2
-
 Coprinus comatus ?
-
 ?
additional information Lac3 exhibits higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes. Mild synergistic decolorization by the laccases isoenzymes Lac 3 and Lac4 is observed for triphenylmethane dyes but not for anthraquinone and azo dyes Coprinus comatus ?
-
 ?
syringaldazine + O2
-
 Coprinus comatus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, isoenzyme Lac4 Coprinus comatus
? x * 75000, isoenzyme Lac3 Coprinus comatus

Synonyms

Synonyms Comment Organism
LAC3
-
 Coprinus comatus
Lac4
-
 Coprinus comatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate: guaiacol, isoenzyme Lac3 Coprinus comatus
60
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac4 Coprinus comatus
60
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac3 Coprinus comatus
60
-
 substrate: guaiacol, isoenzyme Lac4 Coprinus comatus
60
-
 substrate: syringaldazine, isoenzyme Lac3 Coprinus comatus
65
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac3 Coprinus comatus
65
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac4 Coprinus comatus
65
-
 substrate: syringaldazine, isoenzyme Lac4 Coprinus comatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 recombinant laccase is stable at temperatures below 50°C Coprinus comatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.196
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
0.307
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
0.418
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.467
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.468
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
3.485
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
31.96
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
44.71
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac3 Coprinus comatus
3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac4 Coprinus comatus
5
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac3 Coprinus comatus
5
-
 substrate: guaiacol, isoenzyme Lac3 Coprinus comatus
5.5
-
 substrate: syringaldazine, isoenzyme Lac3 Coprinus comatus
5.5
-
 substrate: syringaldazine, isoenzyme Lac4 Coprinus comatus
6
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac4 Coprinus comatus
6
-
 substrate: guaiacol, isoenzyme Lac4 Coprinus comatus

pI Value

Organism Comment pI Value Maximum pI Value
Coprinus comatus isoenzyme Lac3
-
 5.16
Coprinus comatus isoenzyme Lac4
-
 5.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.375
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
0.503
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
7.167
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
25.625
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
219.6
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
367.36
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus