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Literature summary for 1.10.3.2 extracted from

  • Liu, Y.; Huang, L.; Guo, W.; Jia, L.; Fu, Y.; Gui, S.; Lu, F.
    Cloning, expression, and characterization of a thermostable and pH-stable laccase from Klebsiella pneumoniae and its application to dye decolorization (2017), Process Biochem., 53, 125-134 .
No PubMed abstract available
Search for more literature for 1.10.3.2

Application

Application Comment Organism
environmental protection laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.24
-
 2,6-dimethoxyphenol pH 8.0, 70°C Klebsiella pneumoniae
5.33
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 35°C Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 15% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM Klebsiella pneumoniae
Ca2+ about 15% loss of maximal activity at 10 mM, about 25% loss of activity at 50 mM Klebsiella pneumoniae
Cu2+ multicopper enzyme Klebsiella pneumoniae
Mg2+ about 10% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM Klebsiella pneumoniae
Mn2+ about 10% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae 601 MCO
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Klebsiella pneumoniae ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Klebsiella pneumoniae 601 MCO ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Klebsiella pneumoniae ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Klebsiella pneumoniae 601 MCO ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55600, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Klebsiella pneumoniae
70
-
 substrate: 2,6-dimethoxyphenol Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 40 30°C: about 50% of maximal activity, 40°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Klebsiella pneumoniae
45 80 45°C: about 60% of maximal activity, 80°C: about 65% of maximal activity, substrate: 2,6-dimethoxyphenol Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70
-
 Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.02
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 35°C Klebsiella pneumoniae
2.93
-
 2,6-dimethoxyphenol pH 8.0, 70°C Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Klebsiella pneumoniae
8
-
 substrate: 2,6-dimethoxyphenol Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.5 pH 3.5: about 55% of maximal activity, pH 4.5: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Klebsiella pneumoniae
7.5 8.5 pH 7.5: about 45% of maximal activity, pH 8.5: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
 Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 35°C Klebsiella pneumoniae
2.36
-
 2,6-dimethoxyphenol pH 8.0, 70°C Klebsiella pneumoniae