KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.226 | - |
syringaldazine | pH 6.0, 50°C | Trametes flavida | |
0.366 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes flavida | |
0.44 | - |
2,6-dimethoxyphenol | pH 4.5, 37°C | Trametes flavida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes flavida | - |
- |
- |
Trametes flavida MTCC-145 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trametes flavida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes flavida | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes flavida MTCC-145 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes flavida | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes flavida MTCC-145 | ? | - |
? | |
additional information | the enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases | Trametes flavida | ? | - |
? | |
additional information | the enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases | Trametes flavida MTCC-145 | ? | - |
? | |
syringaldazine + O2 | - |
Trametes flavida | ? | - |
? | |
syringaldazine + O2 | - |
Trametes flavida MTCC-145 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Trametes flavida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
substrate: 2,6-dimethoxyphenol | Trametes flavida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the enzyme is most stable at 45°C when exposed for 1 h | Trametes flavida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.45 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes flavida | |
6.45 | - |
2,6-dimethoxyphenol | pH 4.5, 37°C | Trametes flavida | |
6.45 | - |
syringaldazine | pH 6.0, 50°C | Trametes flavida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
substrate: 2,6-dimethoxyphenol | Trametes flavida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
the enzyme is most stable at pH 5.0 when exposed for 1 h | Trametes flavida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.7 | - |
2,6-dimethoxyphenol | pH 4.5, 37°C | Trametes flavida | |
17.6 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes flavida | |
28.5 | - |
syringaldazine | pH 6.0, 50°C | Trametes flavida |