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Literature summary for 1.10.3.2 extracted from

  • Sharma, M.; Chaurasia, P.; Yadav, A.; Yadav, R.; Yadava, S.; Yadav, K.
    Purification and characterization of a thermally stable yellow laccase from Daedalea flavida MTCC-145 with higher catalytic performance towards selective synthesis of substituted benzaldehydes (2016), Russ. J. Bioorg. Chem., 42, 59-68 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.226
-
syringaldazine pH 6.0, 50°C Trametes flavida
0.366
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes flavida
0.44
-
2,6-dimethoxyphenol pH 4.5, 37°C Trametes flavida

Organism

Organism UniProt Comment Textmining
Trametes flavida
-
-
-
Trametes flavida MTCC-145
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trametes flavida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Trametes flavida ?
-
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Trametes flavida MTCC-145 ?
-
?
2,6-dimethoxyphenol + O2
-
Trametes flavida ?
-
?
2,6-dimethoxyphenol + O2
-
Trametes flavida MTCC-145 ?
-
?
additional information the enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases Trametes flavida ?
-
?
additional information the enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases Trametes flavida MTCC-145 ?
-
?
syringaldazine + O2
-
Trametes flavida ?
-
?
syringaldazine + O2
-
Trametes flavida MTCC-145 ?
-
?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Trametes flavida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
substrate: 2,6-dimethoxyphenol Trametes flavida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
the enzyme is most stable at 45°C when exposed for 1 h Trametes flavida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.45
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes flavida
6.45
-
2,6-dimethoxyphenol pH 4.5, 37°C Trametes flavida
6.45
-
syringaldazine pH 6.0, 50°C Trametes flavida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
substrate: 2,6-dimethoxyphenol Trametes flavida

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
the enzyme is most stable at pH 5.0 when exposed for 1 h Trametes flavida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.7
-
2,6-dimethoxyphenol pH 4.5, 37°C Trametes flavida
17.6
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes flavida
28.5
-
syringaldazine pH 6.0, 50°C Trametes flavida