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Literature summary for 1.10.3.2 extracted from

  • Kumari, A.; Kishor, N.; Guptasarma, P.
    Characterization of a mildly alkalophilic and thermostable recombinant Thermus thermophilus laccase with applications in decolourization of dyes (2018), Biotechnol. Lett., 40, 285-295 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
industry the enzyme decoloures up to six different industrial dyes, with or without the use of redox mediators such as ABTS Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0153
-
 syringaldazine 40°C, pH 6.0 Thermus thermophilus
0.167
-
 syringaldazine 35°C, pH 6.0 Thermus thermophilus
0.256
-
 syringaldazine 30°C, pH 6.0 Thermus thermophilus
2.4
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 60°C, pH 6.0 Thermus thermophilus
3.8
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 55°C, pH 6.0 Thermus thermophilus
4.4
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 50°C, pH 6.0 Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 SDS-PAGE Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72HW2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Thermus thermophilus ?
-
 ?
4-fluoro-2-methylphenol + O2
-
 Thermus thermophilus ?
-
 ?
additional information the purified enzyme is able to decolourize Green Dye, Orange Dye and Acid Red Dye. The enzyme is unable to decolourize NBB, RBB, or Congo Red. However, with 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) (5 mM) acting as redox mediator, the enzyme decolourizes NBB, RBB, or Congo Red Thermus thermophilus ?
-
 ?
syringaldazine + O2
-
 Thermus thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 5300, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
TthLAC
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 reaction time: 180 min Thermus thermophilus
90
-
 reactions time: less than 1 h Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 85 40°C: about 40% of maximal activity, 85°C: about 55% of maximal activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the enzyme undergoes slow partial unfolding and thermal inactivation above 65°C, making it unsuitable for long incubations above this temperature Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.58
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 50°C, pH 6.0 Thermus thermophilus
3.33
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 55°C, pH 6.0 Thermus thermophilus
3.66
-
 syringaldazine 30°C, pH 6.0 Thermus thermophilus
4.77
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 60°C, pH 6.0 Thermus thermophilus
5.33
-
 syringaldazine 35°C, pH 6.0 Thermus thermophilus
10.3
-
 syringaldazine 40°C, pH 6.0 Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Thermus thermophilus