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Literature summary for 1.10.3.2 extracted from

  • Kataoka, K.; Komori, H.; Ueki, Y.; Konno, Y.; Kamitaka, Y.; Kurose, S.; Tsujimura, S.; Higuchi, Y.; Kano, K.; Seo, D.; Sakurai, T.
    Structure and function of the engineered multicopper oxidase CueO from Escherichia coli - deletion of the methionine-rich helical region covering the substrate-binding site (2007), J. Mol. Biol., 373, 141-152 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the region Pro357-His406 deletion mutant, to 1.81 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of the region Pro357-His406 comprising a methionine-rich helical segment that covers the substrate-binding site and replacement with a Gly-Gly linker. The scaffold of the CueO molecule and metal-binding sites are reserved in the mutant. The high thermostability of the protein molecule and its spectroscopic and magnetic properties are also conserved after truncation. The cuprous oxidase activity of the mutant is reduced to about 10% that of recombinant CueO due to the decrease in the affinity of the labile Cu site for Cu(I) ions Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 2,6-dimethoxyphenol region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
2.5
-
 2,6-dimethoxyphenol wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
6.4
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
6.5
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, pH 5.5, 25°C Escherichia coli
11
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
12
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) region Pro357-His406 deletion mutant, pH 5.5, 25°C Escherichia coli
90
-
 p-phenylenediamine region Pro357-His406 deletion mutant, pH 5.5, 25°C Escherichia coli
375
-
 p-phenylenediamine wild-type, pH 5.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P36649 bifunctional copper oxidase and laccase, cf. EC 1.16.3.4
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, pH 5.5, 25°C Escherichia coli
0.53
-
 substrate p-phenylendiamine, wild-type, pH 5.5, 25°C Escherichia coli
4.6
-
 substrate p-phenylendiamine, region Pro357-His406 deletion mutant, pH 5.5, 25°C Escherichia coli
12
-
 substrate catechol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
15
-
 substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, pH 5.5, 25°C Escherichia coli
16
-
 substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
19
-
 substrate p-phenylendiamine, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
20
-
 substrate p-phenylendiamine, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
29
-
 substrate 2,6-dimethoxyphenol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
30
-
 substrate 2,6-dimethoxyphenol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
34
-
 substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli
52
-
 substrate catechol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Escherichia coli ?
-
 ?
2,6-dimethoxyphenol + H2O
-
 Escherichia coli ?
-
 ?
catechol + O2
-
 Escherichia coli 1,2-benzoquinone + H2O
-
 ?
p-phenylenediamine + O2
-
 Escherichia coli ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CueO
-
 Escherichia coli
YacK
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 above 75°C, both wild-type and region Pro357-His406 deletion mutant Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, no loss of activity, both wild-type and region Pro357-His406 deletion mutant Escherichia coli
60
-
 30 min, 50% loss of activity, both wild-type and region Pro357-His406 deletion mutant Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 region Pro357-His406 deletion mutant Escherichia coli
8
-
 wild-type Escherichia coli